Application of Polymerase Chain Reaction–Single Strand Conformational Polymorphism (PCR–SSCP) to Identification of Flatfish Species
1999; Oxford University Press; Volume: 82; Issue: 4 Linguagem: Inglês
10.1093/jaoac/82.4.903
ISSN1944-7922
AutoresAna Céspedes, Teresa Garcı́a, Esther Carrera, Isabel González, Esther Carrera, Alicia Fernández, Pablo E. Hernández, Rosario Martı́n,
Tópico(s)Identification and Quantification in Food
ResumoA method of DNA analysis based on polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).
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