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Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report

1996; Elsevier BV; Volume: 65; Issue: 5 Linguagem: Inglês

10.1016/s0015-0282(16)58285-5

1556-5653

Z.P. Nagy, Janssenwillen Cecile, Jiaen Liu, Ann Loccufier, Paul Devroey, André Van Steirteghem,

Ovarian function and disorders

To report a normal pregnancy and the delivery of a healthy child after the combination of in vitro maturation of germinal-vesicle stage oocytes and intracytoplasmic sperm injection (ICSI) in a patient.Procedures were performed in a tertiary IVF center coupled with an institutional research environment.Maturation rate of immature oocytes after in vitro maturation and intactness, fertilization, and developmental rates of oocytes after microinjection.Nine of 14 germinal-vesicle stage oocytes matured to the metaphase II stage after 30 hours of in vitro culture (64%). Seven of eight injected and intact oocytes fertilized normally (78%) and five of them cleaved with < 20% fragmentation (71%). Four embryos were transferred and a singleton pregnancy was obtained that ended in the delivery of a healthy child.In vitro maturation of immature oocytes together with ICSI can result in normal fertilization, embryo development, pregnancy, and the delivery of healthy child.