Platelet aggregation and exposure of fibrinogen receptors by prostaglandin endoperoxide analogues
1983; Elsevier BV; Volume: 61; Issue: 1 Linguagem: Inglês
10.1182/blood.v61.1.41.41
ISSN1528-0020
AutoresTA Morinelli, Stefan Niewiarowski, Elizabeth Kornecki, WR Figures, Y T Wachtfogel, RW Colman,
Tópico(s)Heparin-Induced Thrombocytopenia and Thrombosis
ResumoTwo stable analogues of prostaglandin endoperoxides-9.1 1 -azo-prostanoid III (9-1 1 -azo PGH2) and 9.1 1 -methanoepoxy PGH2 (U4661 9 compound)-caused aggregation of washed human platelets and release of a-granule contents.Platelet aggregation induced by these compounds was potentiated by the addition of purified human fibrinogen in a dose-dependent manner.Incubation of prostaglandin endoperoxide analogues with washed platelets in a nonstirred system exposed fibrinogen receptors.Enzymes that remove adenosine diphosphate (ADP) [apyrase and creatine phosphate in the presence of creatine phosphokinase (CP/CPK)J.adenosine triphosphate (ATP).a competitive antagonist of ADP. and 5' parafluorosulfonylbenzoyl adenosine (FSBA).an affinity label for ADP binding sites.all blocked platelet aggregation and binding of '25l-fibrinogen to platelets stimulated by prostaglandin endoperoxide ana-Iogues.In contrast.these compounds did not prevent platelet shape change induced by prostaglandin endoper-
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