Portal de Periódicos da CAPES

The Expression of Epidermal Growth Factor Receptor on Human Bladder Cancer: Potential Use in Radioimmunoscintigraphy

1991; Lippincott Williams & Wilkins; Volume: 146; Issue: 1 Linguagem: Inglês

10.1016/s0022-5347(17)37757-1

1527-3792

J. Harney, Monica Liebert, Gary Wedemeyer, Raymond Washington, Judith Stein, Donald J. Buchsbaum, Zenon Steplewski, H. Barton Grossman,

Glycosylation and Glycoproteins Research

No AccessJournal of Urology1 Jul 1991The Expression of Epidermal Growth Factor Receptor on Human Bladder Cancer: Potential Use in Radioimmunoscintigraphy J.V. Harney, M. Liebert, G. Wedemeyer, R. Washington, J. Stein, D. Buchsbaum, Z. Steplewski, and H.B. Grossman J.V. HarneyJ.V. Harney More articles by this author , M. LiebertM. Liebert More articles by this author , G. WedemeyerG. Wedemeyer More articles by this author , R. WashingtonR. Washington More articles by this author , J. SteinJ. Stein More articles by this author , D. BuchsbaumD. Buchsbaum More articles by this author , Z. SteplewskiZ. Steplewski More articles by this author , and H.B. GrossmanH.B. Grossman More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)37757-1AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Monoclonal antibody 425, which binds to an extracellular domain of the epidermal growth factor receptor, was used to evaluate the expression of this antigen on bladder cancer cells. Epidermal growth factor receptor was found on all bladder cancer cell lines tested. Immunoperoxidase staining of fourteen Invasive human bladder cancers with monoclonal antibody 425 demonstrated that ten showed strong staining, one showed weak staining and three were negative. Five non-invasive tumors were similarly examined. Four of these were negative and one showed weak staining. Biodistribution experiments with human bladder tumor xenografts in athymic nude mice using radiolabeled monoclonal antibody 425 and an isotype matched control antibody demonstrated specific tumor localization at five and seven days following antibody injection. Successful imaging of a human bladder tumor xenograft was achieved five days post antibody injection. These data confirm that epidermal growth factor receptor expression correlates with bladder cancer stage and suggests that epidermal growth factor receptor may serve as a target antigen for radioimmunoscintigraphy. © 1991 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited BySandberg A and Berger C (2018) Review of Chromosome Studies in Urological Tumors. II. Cytogenetics and Molecular Genetics of Bladder CancerJournal of Urology, VOL. 151, NO. 3, (545-560), Online publication date: 1-Mar-1994. Volume 146Issue 1July 1991Page: 227-231 Advertisement Copyright & Permissions© 1991 by The American Urological Association Education and Research, Inc.Keywordsepidermal growth factorantibodiesneoplasmsmonoclonalbladderMetricsAuthor Information J.V. Harney More articles by this author M. Liebert More articles by this author G. Wedemeyer More articles by this author R. Washington More articles by this author J. Stein More articles by this author D. Buchsbaum More articles by this author Z. Steplewski More articles by this author H.B. Grossman More articles by this author Expand All Advertisement Loading ...