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The Pepsins of Human Gastric Juice

1974; Taylor & Francis; Volume: 9; Issue: 8 Linguagem: Inglês

10.1080/00365521.1974.12096900

1502-7708

D. P. Whitecross, A. D. Clarke, D. W. Piper,

Biochemical Analysis and Sensing Techniques

Whitecross, D. P., Clarke, A. D. & Piper, D. W. The pepsins of human gastric juice. Separation by electrophoresis and chromatography; the influence of gastric disease and ambient pH. Scand. J. Gastroent. 1974, 9, 711–717.Acid proteases found in human gastric juice have been separated into four or five proteases either by high voltage electrophoresis or by column chromatography using DEAE cellulose. The chromatographic fractions are pure on gel electrophoresis. The pH of the ambient secreted gastric juice influences the number of pepsins obtained; if the pH is less than 2.5, five bands are found, whereas if greater than 2.5, four bands are present. The pattern is uninfluenced by the presence of peptic ulceration.