Cell age optimization for hydrogen production induced by sulfur deprivation using a green alga Chlamydomonas reinhardtii utex 90
2005; Springer Science+Business Media; Volume: 15; Issue: 1 Linguagem: Inglês
ISSN
1738-8872
AutoresJun Pyo Kim, Chang Duk Kang, Sang Jun Sim, Mi Sun Kim, Tai Hyun Park, Donghyun Lee, Dukjoon Kim, Ji‐Heung Kim, Youngkwan Lee, Daewon Pak,
Tópico(s)Photosynthetic Processes and Mechanisms
ResumoUnder sulfur deprived conditions, PS II and photosynthetic O 2 evolution by Chlamydomonas reinhardtii UTEX 90 are inactivated, resulting in shift from aerobic to anaerobic condition. This is followed by hydrogen production catalyzed by hydrogenase. We hypothesized that the photosynthetic capacity and the accumulation of endogenous substrates such as starch for hydrogen production might be different according to cell age. Accordingly, we investigated (a) the relationships between hydrogen production, induction time of sulfur deprivation, increase of chlorophyll after sulfur deprivation, and residual PS II activity, and (b) the effect of initial cell density upon sulfur deprivation. The maximum production volume of hydrogen was 151 ml H 2 /l with 0.91 g/l of cell density in the late-exponential phase. We suggest that the effects of induction time and initial cell density at sulfur deprivation on hydrogen production, up to an optimal concentration, are due to an increase of chlorophyll under sulfur deprivation.
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