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Genomic Approaches for Interrogating the Biochemistry of Medicinal Plant Species

2012; Academic Press; Linguagem: Inglês

10.1016/b978-0-12-404634-4.00007-3

1557-7988

Elsa Góngora‐Castillo, Greg Fedewa, Yunsoo Yeo, Joe Chappell, DellaPenna Dean, C. Robin Buell,

Plant biochemistry and biosynthesis

Development of next-generation sequencing, coupled with the advancement of computational methods, has allowed researchers to access the transcriptomes of recalcitrant genomes such as those of medicinal plant species. Through the sequencing of even a few cDNA libraries, a broad representation of the transcriptome of any medicinal plant species can be obtained, providing a robust resource for gene discovery and downstream biochemical pathway discovery. When coupled to estimation of expression abundances in specific tissues from a developmental series, biotic stress, abiotic stress, or elicitor challenge, informative coexpression and differential expression estimates on a whole transcriptome level can be obtained to identify candidates for function discovery.