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Identification of proliferative progenitors associated with prominent postnatal growth of the pons

2016; Nature Portfolio; Volume: 7; Issue: 1 Linguagem: Inglês

10.1038/ncomms11628

2041-1723

Robert A. Lindquist, Cristina D. Guinto, Jose L. Rodas-Rodriguez, Luis C. Fuentealba, Matthew C. Tate, David H. Rowitch, Arturo Álvarez-Buylla,

Congenital heart defects research

Abstract The pons controls crucial sensorimotor and autonomic functions. In humans, it grows sixfold postnatally and is a site of paediatric gliomas; however, the mechanisms of pontine growth remain poorly understood. We show that the murine pons quadruples in volume postnatally; growth is fastest during postnatal days 0–4 (P0–P4), preceding most myelination. We identify three postnatal proliferative compartments: ventricular, midline and parenchymal. We find no evidence of postnatal neurogenesis in the pons, but each progenitor compartment produces new astroglia and oligodendroglia; the latter expand 10- to 18-fold postnatally, and are derived mostly from the parenchyma. Nearly all parenchymal progenitors at P4 are Sox2 + Olig2 + , but by P8 a Sox2 − subpopulation emerges, suggesting a lineage progression from Sox2 + ‘early’ to Sox2 − ‘late’ oligodendrocyte progenitor. Fate mapping reveals that >90% of adult oligodendrocytes derive from P2–P3 Sox2 + progenitors. These results demonstrate the importance of postnatal Sox2 + Olig2 + progenitors in pontine growth and oligodendrogenesis.