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Comparison of LDPI to SPECT perfusion imaging using 99mTc-sestamibi and 99mTc-pyrophosphate in a murine ischemic hind limb model of neovascularization

2016; Springer Science+Business Media; Volume: 6; Issue: 1 Linguagem: Inglês

10.1186/s13550-016-0199-2

2191-219X

Geert Hendrikx, Mark H. M. Vries, Matthias Bauwens, Marijke De Saint‐Hubert, Allard Wagenaar, Joël Guillaume, Levinia Boonen, Mark J. Post, Felix M. Mottaghy,

Pain Management and Treatment

We aimed to determine the accuracy of laser Doppler perfusion imaging (LDPI) in an animal model for hind limb ischemia.We used a murine (C57Bl/6 mice) ischemic hind limb model in which we compared LDPI with the clinically used (99m)Tc-sestamibi SPECT perfusion imaging (n = 7). In addition, we used the SPECT tracer (99m)Tc-pyrophosphate ((99m)Tc-PyP) to image muscular damage (n = 6).LDPI indicated a quick and prominent decrease in perfusion immediately after ligation, subsequently recovering to 21.9 and 25.2 % 14 days later in the (99m)Tc-sestamibi and (99m)Tc-PyP group, respectively. (99m)Tc-sestamibi SPECT scans also showed a quick decrease in perfusion. However, nearly full recovery was reached 7 days post ligation. Muscular damage, indicated by the uptake of (99m)Tc-PyP, was highest at day 3 and recovered to baseline levels at day 14 post ligation. Postmortem histology supported these findings, as a significantly increased collateral diameter was found 7 and 14 days after ligation and peak macrophage infiltration and TUNEL positivity was found on day 3 after ligation.Here, we indicate that LDPI strongly underestimates perfusion recovery in a hind limb model for profound ischemia.