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Protocol for Inducing Flower Color Somaclonal Variation in Torenia (Torenia fournieri Lind.)

2012; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-62703-074-8_34

1940-6029

Dương Tấn Nhựt, Nguyễn Thanh Hải, Pham Thi Minh Thu, Nguyen Thi, Truong Thi Dieu Hien, Trần Trọng Tuấn, Nguyễn Bá Nam, Nguyễn Phúc Huy, Hoang Xuan Chien, S. Mohan Jain,

Plant Reproductive Biology

White or light purple flower color Torenia (Torenia fournieri Lind.) varieties were successfully developed from the parental variety having violet flowers. This was accomplished by reducing Fe micronutrient in the culture media for the induction of in vitro flowering. The flower induction was highest in modified Murashige and Skoog (MS) medium containing ½ strength of macroelements, microelements, organic additives, and full Fe (M1) when compared to MS medium containing ½ strength of macronutrients, micronutrients, full Fe, and full organic additives (M2). The flower color was stable in two new Torenia varieties through three generations ex vitro. The results showed a wide range of somaclonal variation in flower colors; early flowering occurred in MS medium containing ½ strength of macroelements, microelements, Fe, and full strength of organic additives (M3). The selection of desirable somaclones and their micropropagation in subsequent generations led to the development of new and stable Torenia lines.