Bovine Herpes Virus Expressing Envelope Protein(E2) of Bovine Viral Diarrhea Virus As a Vaccine Candidate.
1999; Japanese Society of Veterinary Science; Volume: 61; Issue: 4 Linguagem: Inglês
10.1292/jvms.61.395
ISSN1347-7439
AutoresChang-Hee Kweon, Seung-Won Kang, Eun‐Jin Choi, Yung-Bai Kang,
Tópico(s)Animal Disease Management and Epidemiology
ResumoThe gene encoding the envelope protein (E2) of bovine viral diarrhea virus (BVDV) was expressed under the thymidine kinase (TK) promoter of Korean bovine herpesvirus 1 (BHV-1) isolate. Thymidine kinase negative (TK-) BHV-1 recombinants expressing E2 of BVDV were constructed and the expression of E2 was identified by immunofluorescence and Western blotting. Compared to wild type BHV-1, the recombinant BHV-1 had a delayed cytopathogenic effect in cells. The immunogenicity of the recombinant BHV-1 was examined in guinea pigs and cattle. Although an increase in body temperature was detected for a few days, the inoculated cattle returned to normal temperature with the development of neutralizing antibodies to BVDV.
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