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Development and Validation of an HPLC-DAD Analytical Procedure for Quality Control of Damiana (Turnera diffusa), Using an Antioxidant Marker Isolated from the Plant

2010; Oxford University Press; Volume: 93; Issue: 4 Linguagem: Inglês

10.1093/jaoac/93.4.1161

1944-7922

Jonathan Pérez-Meseguer, Aurora de Jesús Garza-Juárez, Ricardo Salazar‐Aranda, Ma Luz Salazar-Cavazos, Yael C dela Torre RodríGuez, Verónica M. Rivas-Galindo, Noemí Waksman de Torres,

Phytochemicals and Antioxidant Activities

Abstract Damiana is a shrub used as a dietary supplement and as an additive in several foods. This report presents the first validated analytical method for QC of products made from damiana. Through bioassay-guided fractionation, the compound showing the best antioxidant activity, determined using 2,2-diphenyl-1-picryl-hydrazyl assay in TLC, was purified and identified as 8-C--[6-deoxy-2-O-(-1-rhamnopyranosyl)-xylo- hexopyranos-3-uloside] (CI50 5.56 g/mL). This is the only natural source from which this compound has been reported, and has been found in all native damiana samples analyzed. Therefore, it was used as a biomarker to develop an HPLC-diode-array detection analytical procedure using the first-order derivative of the chromatograms. Separation was achieved using an AccQ Tag C18 150 3.9 mm (4 m) column, with a gradient of methanol and 0.1 trifluoroacetic acid in water. The method was shown to be accurate, sensitive, and reproducible, providing a useful quantitative means to apply QC to damiana products.