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Detection of Onco-Fetal Bladder Antigen in Urine of Patients with Transitional Cell Carcinoma

1987; Lippincott Williams & Wilkins; Volume: 137; Issue: 6 Linguagem: Inglês

10.1016/s0022-5347(17)44477-6

1527-3792

Brian C.‐S. Liu, Harry Neuwirth, Li Wei Zhu, Lawrence M. Stock, Jean B. deKernion, John L. Fahey,

Galectins and Cancer Biology

No AccessJournal of Urology1 Jun 1987Detection of Onco-Fetal Bladder Antigen in Urine of Patients with Transitional Cell Carcinoma Brian C.-S. Liu, Harry Neuwirth, Li Wei Zhu, Lawrence M. Stock, Jean B. Dekernion, and John L. Fahey Brian C.-S. LiuBrian C.-S. Liu More articles by this author , Harry NeuwirthHarry Neuwirth More articles by this author , Li Wei ZhuLi Wei Zhu More articles by this author , Lawrence M. StockLawrence M. Stock More articles by this author , Jean B. DekernionJean B. Dekernion More articles by this author , and John L. FaheyJohn L. Fahey More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)44477-6AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Studies of antigens associated with transitional cell carcinoma were extended by using murine IgM monoclonal antibody E7, developed earlier by this laboratory. These antibodies react preferentially with human bladder tumors and transitional cell carcinoma (TCC) cell line 647V. We now report that monoclonal antibody E7 detected the presence of antigen in midgestational and third trimester amniotic fluids, and in urine of patients with advanced transitional cell carcinoma. Western blot analysis showed that the antigen present in amniotic fluids consists of a sharp band with molecular weight greater than 200 kdaltons. A similar molecular weight pattern was seen with the solubilized membrane of 647V. A sensitive and convenient sandwich ELISA was developed and the urine of patients with bladder cancer was assayed for the presence of the E7 antigen. Antigen was detected in the urine of patients with advanced transitional cell carcinoma but not in the urine of normal adults or in urine from patients with prostate cancer, renal cell carcinoma, or benign prostate hyperplasia. An inhibition enzyme immunoassay was developed with monomeric forms of the E7 antibody and confirmed the presence of antigen in the urine of patients with TCC. We conclude that the E7 antigen is an onco-fetal antigen expressed in patients with transitional cell carcinoma of the bladder. © 1987 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byPlanz B, Striepecke E, Jakse G and Bocking A (2018) USE OF LEWIS X ANTIGEN AND DEOXYRIBONUCLEIC ACID IMAGE CYTOMETRY TO INCREASE SENSITIVITY OF URINARY CYTOLOGY IN TRANSITIONAL CELL CARCINOMA OF THE BLADDERJournal of Urology, VOL. 159, NO. 2, (384-388), Online publication date: 1-Feb-1998.Decken K, Schmitz-Dräger B, Rohde D, Nakamura S, Ebert T and Ackermann R (2018) Monoclonal Antibody Due ABC 3 Directed against Transitional Cell Carcinoma. I. Production, Specificity Analysis, and Preliminary Characterization of the AntigenJournal of Urology, VOL. 147, NO. 1, (235-241), Online publication date: 1-Jan-1992.Sheinfeld J, Reuter V, Melamed M, Fair W, Morse M, Sogani P, Herr H, Whitmore W and Cordon-Cardo C (2018) Enhanced Bladder Cancer Detection with the Lewis X Antigen as a Marker of Neoplastic TransformationJournal of Urology, VOL. 143, NO. 2, (285-288), Online publication date: 1-Feb-1990. Volume 137Issue 6June 1987Page: 1258-1261 Advertisement Copyright & Permissions© 1987 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Brian C.-S. Liu More articles by this author Harry Neuwirth More articles by this author Li Wei Zhu More articles by this author Lawrence M. Stock More articles by this author Jean B. Dekernion More articles by this author John L. Fahey More articles by this author Expand All Advertisement PDF downloadLoading ...