Broad-Host-Range Plasmid Cloning Vectors for Gram-Negative Bacteria
1988; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-409-90042-2.50021-0
AutoresThomas J. Schmidhauser, Gary S. Ditta, Donald R. Helinski,
Tópico(s)Escherichia coli research studies
ResumoThe wealth of genetic information available for the enteric bacterium Escherichia coli has led to the development of sophisticated vectors for gene cloning and expression in this bacterium. However, there are many gram-negative bacteria of practical and theoretical importance for which these vectors are of limited use. Five plasmid incompatibility groups, IncC, IncN, IncP-1, IncQ, and IncW, are known for broad-host-range maintenance properties. Plasmids from three of these five groups, that isIncP-1, IncQ, and IncW have been modified for use as cloning vectors. This chapter describes the progress in modifying broad-host-range plasmids as vectors for gene cloning in bacteria. The limited numbers of broad-host-range replicons available for vector development are explained in the chapter. The A few vector properties are—(1) copy number, host range, and stability of replicon maintenance; (2) restriction site availability and selectable or screenable markers; (3) utility in the construction of gene banks; (4) ease of establishing multiple plasmid vectors in the same cell; and (5) capabilities for gene fusions.
Referência(s)