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BIBTEX RIS

Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents

2016; Instituto Oswaldo Cruz, Ministério da Saúde; Volume: 111; Issue: 5 Linguagem: Inglês

10.1590/0074-02760150412

ISSN

1678-8060

Autores

Erika Verissimo-Villela, Milene Yoko Kitahara-Oliveira, Ana Beatriz de Bragança dos Reis, Rodolpho Mattos Albano, Alda Maria Da‐Cruz, Alexandre Ribeiro Bello,

Tópico(s)

Insect Resistance and Genetics

Resumo

During its life cycle Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living organisms. DNA repair in trypanosomatids has been reported only for Old World Leishmania species. Here the AP endonuclease from Leishmania (L.) amazonensis was cloned, expressed in Escherichia coli mutants defective on the DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that LAMAP belongs to the AP endonuclease class of proteins.

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