UNCONVENTIONAL SUBSTRATES FOR IN VITRO ROOTING OF REBUTIA
2010; International Society for Horticultural Science; Issue: 865 Linguagem: Inglês
10.17660/actahortic.2010.865.36
ISSN2406-6168
AutoresA. Medrano Valverde, D. Orea Coria,
Tópico(s)Botanical Research and Applications
ResumoThe objective of this investigation was to know the suitability of three nonconventional substrates for the in vitro rooting of plantlets of Rebutia cactus in comparison of agar, with the purpose of finding a cheaper and appropriate substitute for the in vitro rooting stage and transplant to the phase of acclimatization. Three substrates as well as agar were proved: perlite, pumice stone and luffa sponge. Rebutia micropropagated plantlets were transferred to MS half-strength medium containing 5.37 μM NAA. Roots occurrence was evaluated after 6 weeks in cultivation. Roots were produced rapidly and more vigorous in treatments with luffa sponge and agar. The treatments with perlite and pumice stone behaved less efficiently. No morphological alteration was observed during the plantlet culture in the alternative substrates. It is easy to notice the rooting process in agar, pumice stone and luffa sponge. With the use of perlite this observation becomes difficult and only the roots are visible when they reach the surface of the culture container. Luffa sponge represents an acceptable alternative for in vitro rooting for this species.
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