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Stochastic sensors designed for assessment of biomarkers specific to obesity

2016; Elsevier BV; Volume: 128; Linguagem: Inglês

10.1016/j.jpba.2016.05.040

1873-264X

Catalina Cioates Negut, Raluca‐Ioana Stefan‐van Staden, Eleonora‐Mihaela Ungureanu, Denisa Ioana Udeanu,

Biochemical Analysis and Sensing Techniques

Two stochastic sensors based on the following oleamides: 1-adamantyloleamide and N,N-dimethyl-N-(2-oleylamidoethyl)amine physically immobilized on graphite paste were designed. The sensors were able to determine simultaneously from the whole blood of Wistar rats three biomarkers specific to obesity: leptin, interleukin-6 (IL-6) and plasminogen activator inhibitor 1 (PAI-1). The whole blood samples were obtained from Wistar rats treated with oleoylethanolamide (OEA), (Z)-N-[(1S)-2-hidroxy-1-(phenylmethyl) ethyl]-9octadecenamide (OLA), and with the aqueous solution of 1% Tween 80 used as solvent for oleamides formulations (control samples). The proposed sensors were very sensitive and reliable for the assay of obesity biomarkers in whole blood of rats.