Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol.
1979; Elsevier BV; Volume: 20; Issue: 7 Linguagem: Inglês
10.1016/s0022-2275(20)40010-0
ISSN1539-7262
Autores Tópico(s)Lipid metabolism and biosynthesis
ResumoThe interaction of sonicated, small vesicles of egg phosphatidylcholine and cholesterol (2: 1, mol/mol) with bovine high density serum lipoproteins was examined in terms of lipid transfer between both types of particles and the resulting changes in lipoprotein structure.Saturation of high density lipoprotein preparations with vesicle lipids gave final lipoprotein particles with essentially unchanged protein content and composition, unchanged cholesteryl ester and nonpolar lipid content, but with markedly in- creased phospholipid content (59% increase by weight) and moderately increased cholesterol content (20% increase by weight).The lipoproteins enriched in lipid were rela-Abbreviations: HDL, high density lipoprotein (d 1.063-1.170g/ml); PC, phosphatidylcholine; DPH, 1,6-diphenyI-1,3,5-hexatriene, fluorescent lipophilic probe; apoA-I, apolipoprotein A-I, the major protein component of HDL; PL, phospholipid.
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