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Porcine Spermatozoan Respiration and Motility in the Presence of Bovine Follicular Fluid

1975; Oxford University Press; Volume: 40; Issue: 1 Linguagem: Inglês

10.2527/jas1975.40196x

1544-7847

H. E. Grotjan, Billy N. Day, Dennis T. Mayer,

Animal Genetics and Reproduction

Respiration and motility of washed porcine spermatozoa in the presence of bovine follicular fluid were studied. A Gilson Differential Respirometer was used to measure respiration rates (ZO2 values). Final motility scores were visually estimated using a microscope. Whole bovine follicular fluid (WBFF) as 5, 10 or 20% of the suspension medium had a detrimental effect (P< .05) on respiration rates and motility as well as caused head-to-head agglutination relative to spermatozoa suspended entirely in Ca++ -free Krebs-Ringer phosphate buffer (KRPG) containing 1 mg glucose per milliliter. Dialyzed (DBFF) or heated (30 min at 60 C) bovine follicular fluid (HBFF) was less toxic to spermatozoa than WBFF. Spermatozoa suspended in 20% HBFF exhibited significantly higher (P< .05) respiration rates than KRPG suspended controls.