Recommendations for the Implementation of BRCA Testing in the Care and Treatment Pathways of Ovarian Cancer Patients
2016; Future Medicine; Volume: 12; Issue: 18 Linguagem: Inglês
10.2217/fon-2016-0189
ISSN1744-8301
AutoresCarmine Pinto, Maria Angela Bella, Ettore Capoluongo, Paola Carrera, Claudio Clemente, Nicoletta Colombo, Laura Cortesi, Gaetano De Rosa, Francesca Fenizia, Maurizio Genuardi, Stefania Gori, Valentina Guarneri, Antonio Marchetti, Paolo Marchetti, Nicola Normanno, Barbara Pasini, Sandro Pignata, Paolo Radice, Enrico Ricevuto, Antonio Russo, Pierosandro Tagliaferri, Pierfrancesco Tassone, Mauro Truini, Liliana Varesco,
Tópico(s)DNA Repair Mechanisms
ResumoFuture OncologyVol. 12, No. 18 CommentaryRecommendations for the implementation of BRCA testing in the care and treatment pathways of ovarian cancer patientsCarmine Pinto, Maria Angela Bella, Ettore Capoluongo, Paola Carrera, Claudio Clemente, Nicoletta Colombo, Laura Cortesi, Gaetano De Rosa, Francesca Fenizia, Maurizio Genuardi, Stefania Gori, Valentina Guarneri, Antonio Marchetti, Paolo Marchetti, Nicola Normanno, Barbara Pasini, Sandro Pignata, Paolo Radice, Enrico Ricevuto, Antonio Russo, Pierosandro Tagliaferri, Pierfrancesco Tassone, Mauro Truini & Liliana VarescoCarmine Pinto*Author for correspondence: E-mail Address: pinto.carmine@asmn.re.it Medical Oncology S.Maria Nuova Hospital – IRCCS Reggio Emilia, Italy, Maria Angela Bella Medical Oncology Unit, University Hospital of Parma, Italy, Ettore Capoluongo Institute of Biochemistry & Clinical Biochemistry, Catholic University of the Sacred Heart, Rome, Italy, Paola Carrera Unit of Genomics for Diagnosis of Human Pathologies, Division of Genetics & Cell Biology, & Laboratory of Clinical Molecular Biology, IRCCS Ospedale San Raffaele, Milan, Italy, Claudio Clemente Pathology & Cytopathology Unit, Casa di Cura San Pio X & IRCCS Policlinico San Donato, Milano, Italy, Nicoletta Colombo European Institute of Oncology 'IEO', Milan, Italy, Laura Cortesi Department of Oncology, Hematology & Respiratory Diseases, University Hospital of Modena, Italy, Gaetano De Rosa Department of Advanced Biomedical Sciences, Pathology Section, University of Naples Federico II, Naples, Italy, Francesca Fenizia Pharmacogenomic Laboratory, Centro di Ricerche Oncologiche di Mercogliano (CROM), Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione Giovanni Pascale" – IRCCS Naples, Italy, Maurizio Genuardi Institute of Genomic Medicine, 'A Gemelli' School of Medicine, Catholic University of the Sacred Heart, Rome, Italy, Stefania Gori Department of Oncology, Ospedale Sacro Cuore Don Calabria, Negrar-Verona, Italy, Valentina Guarneri Department of Surgery, Oncology & Gastroenterology, University of Padova; Medical Oncology 2, Istituto Oncologico Veneto IRCCS, Padova, Italy, Antonio Marchetti Center of Predictive Molecular Medicine, University-Foundation, CeSI Biotech Chieti, Italy, Paolo Marchetti Clinical & Molecular Medicine Department, Sapienza University, Rome, Italy, Nicola Normanno Cell Biology & Biotherapy Unit, Istituto Nazionale per lo Studio e la Cura dei Tumori 'Fondazione Giovanni Pascale' – IRCCS Naples, Italy, Barbara Pasini Department of Medical Science, University of Turin, Turin, Italy, Sandro Pignata Uro-Gynecological Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori 'Fondazione Giovanni Pascale' – IRCCS Naples, Italy, Paolo Radice Unit of Molecular Bases of Genetic Risk & Genetic Testing, Department of Preventive & Predictive Medicine, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy, Enrico Ricevuto Oncology Network ASL1 Abruzzo, Oncology Territorial Care Unit, Division of Medical Oncology, Department of Biotechnological & Applied Clinical Sciences, University of L'Aquila, Italy, Antonio Russo Department of Surgical, Oncological & Oral Sciences, Section of Medical Oncology, University of Palermo, Italy, Pierosandro Tagliaferri Department of Experimental & Clinical Medicine, Magna Graecia University, Salvatore Venuta University Campus, Catanzaro, Italy, Pierfrancesco Tassone Sbarro Institute for Cancer Research & Molecular Medicine, Center for Biotechnology, College of Science & Technology, Temple University, Philadelphia, PA, USA, Mauro Truini Pathological Anatomy Histology & Cytogenetics, Niguarda Cancer Center, Niguarda Ca' Granda Hospital, Milan, Italy & Liliana Varesco Unit of Hereditary Cancers, IRCCS AOU San Martino – IST, Genoa, ItalyPublished Online:31 May 2016https://doi.org/10.2217/fon-2016-0189AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInReddit Keywords: BRCA1BRCA2genetic testinggermline mutationsovarian cancerPARP inhibitorssomatic mutationsFirst submitted: 13 April 2016; Accepted for publication: 10 May 2016; Published online: 31 May 2016In the last 20 years, following the identification of the BRCA1 and BRCA2 genes (hereinafter referred to as the BRCA genes), preventive pathways have been developed for the identification and clinical management of individuals at high risk of developing breast and ovarian cancer due to the presence of a pathogenic variant in either of these genes. These pathways are aimed at educating high-risk subjects on programs targeted toward early diagnosis and cancer risk reduction.The approval of a novel class of drugs, the PARP enzyme inhibitors, for the treatment of ovarian cancer patients carrying high-risk BRCA pathogenic variants has changed this scenario. BRCA testing, in addition to providing information on the risk of disease, has become also a predictive marker of drug response in ovarian carcinoma patients. These recommendations prepared by Associazione Italiana di Oncologia Medica (AIOM), Società Italiana Genetica Umana (SIGU), Società Italiana di Biochimica Clinica e Biologia Molecolare Clinica (SIBIOC) and Società Italiana di Anatomia Patologica e Citologia Diagnostica – Italian Division of the International Academy of Pathology (SIAPEC-IAP) are focused on the implementation of BRCA testing in the care and treatment pathways of ovarian cancer patients.BRCA testing for the prediction of treatment efficacyRecent population studies have highlighted that the prevalence of constitutional BRCA pathogenic variants in ovarian cancer patients is >10%, independently of age of diagnosis and of family history of breast/ovarian cancer [1,2]. The prevalence of pathogenic variants progressively increases in patients with serous ovarian carcinoma (17–20%), high-grade serous carcinoma (23–25%) and in platinum-sensitive patients (30–40%) [3–6]. Furthermore, approximately 25% of women carrying pathogenic BRCA variants have a diagnosis of ovarian carcinoma over 60 years of age [1–3].Retrospective studies suggest that heterozygosity for a BRCA hereditary pathogenic variant in ovarian cancer patients is associated with a significantly more favorable prognosis and is predictive of sensitivity to combination therapies containing platinum derivatives [3,7].Somatic mutations of the BRCA genes have been identified in approximately 6% of serous ovarian carcinomas [4,6], thus suggesting that inactivation of these genes can also occur during cancer progression.Importantly, it has been shown that BRCA gene mutations, whether constitutional or somatic, are a biomarker of sensitivity to treatment with PARP inhibitors, a class of pharmacological agents involved in the repair of single-strand DNA breaks, in patients with advanced ovarian cancer [8,9]. The therapeutic effect of PARP inhibitors in ovarian cancer is due to 'synthetic lethality', which occurs in cells with an inactive double-strand DNA repair mechanism mediated by homologous recombination (HR). The BRCA1/2 proteins play an essential role in HR [8–13], and their loss of function due to constitutional or somatic BRCA gene mutations can be one of the major causes of HR dysfunction [6,14–15]. Following clinical trials, the PARP inhibitor olaparib has been registered in October 2014 by EMA as a maintenance therapy in patients with relapsed high-grade serous platinum-sensitive ovarian cancer, Fallopian tube cancer and primary peritoneal cancer [5,9,16].Hence, BRCA testing is now a prerequisite for the indication of PARP inhibitor therapy. However, it is advisable to consider offering the test at the time of initial diagnosis to all patients with nonmucinous and nonborderline ovarian epithelial carcinoma, fallopian tube carcinoma and primary peritoneal carcinoma. This approach would allow us to obtain early the information on the potential sensitivity to PARP inhibitors and at the same time it would also pave the way for cancer genetic counseling and prevention. The timing of BRCA testing should be chosen with the patient in order to respect her needs for the decision-making process. In addition, the patient must be provided with appropriate and thorough information regarding all issues associated with a positive test outcome.Types of BRCA testingThe BRCA test on peripheral blood detects constitutional/hereditary variants – that is, those which can be transmitted to the offspring (50% probability for each child). On the other hand, the test conducted on tumor tissue can also identify the variants acquired by somatic mutations during tumor development and progression, in addition to constitutional defects. This implies that when a variant is identified in tumor DNA, its origin (constitutional or somatic) must be established by analyzing normal tissue (blood or other tissue).According to available data, 2/3 of the pathogenic BRCA variants identified in patients affected by ovarian cancer are constitutional (present in every cell of the individual) and 1/3 somatic (confined to tumor tissue) [4,6]. Constitutional BRCA variants are nearly always inherited from one parent; less than 1% of cases are due to de novo mutations, occurring in the parental germ cells or in utero.In the clinical setting, mutation screening of BRCA1 and BRCA2 is usually carried out by sequencing of all coding exons (n = 22 and n = 26, respectively) and corresponding exon/intron junctions. This allows detection of point variations (single nucleotide substitutions and insertions/deletions of one or a few bases) in the DNA sequence. These alterations encompass more than 90% of BRCA pathogenic variants occurring in the coding regions of both genes. In order to maximize sensitivity, the test must include the search for large genomic rearrangements (i.e., deletions or duplications of one or more exons, or of the whole gene), which account for a variable proportion of constitutional BRCA variants across populations, usually not exceeding 10%. These types of changes can be investigated by multiplex ligation probe dependent amplification or multiplex amplicon quantification [17,18].Currently, sequencing analyses for the detection of constitutional pathogenic BRCA gene variants on peripheral blood is performed in most laboratories using different methods. These include well-established techniques, namely Sanger sequencing, as well as novel technologies – that is, next-generation sequencing, which are under validation [19–24].There are as yet no standardized tests for BRCA analysis in tumor samples. BRCA testing in tumor tissue is more complex than other molecular somatic tests already included in routine cancer diagnosis. This is due to the high degree of heterogeneity of potentially pathogenic variants, which can be observed along the entire sequence of BRCA1 and BRCA2 genes. Furthermore, in addition to the already mentioned different types of DNA variations (point variations, large deletions/duplications), epigenetic alterations (methylation of the regulatory regions) can also occur [4,15]. This makes it difficult to assess the predictive value, in terms of response to treatment, of the different BRCA variants, in particular those identified at somatic level. Indeed, the available classification algorithms have been devised to identify constitutional variants associated with a high risk of developing breast and ovarian cancer (see below). While BRCA mutation test in blood DNA is routinely applied in the search of constitutional pathogenic variants, the actual feasibility of the somatic test by the diagnostic laboratory should be verified in advance, in consideration of the inherent methodological difficulties. Laboratories should carry out technical assessment procedures and take part in external quality control programs of the tests they offer (constitutional and/or somatic) [25].Interpretation of BRCA genetic variantsThe mutation spectrum of the BRCA genes is very broad, and the interpretation of the clinical significance of identified variants is not always straightforward. Indeed, variant classification is an important aspect of the BRCA testing process, particularly when considering that quite often clinical testing detects genetic alterations not reported in the scientific literature [26]. Therefore, although no consolidated standards exist for the classification of constitutional BRCA variants, it is important that laboratories use updated classification criteria, and that reports be written up in accordance with good laboratory practice recommendations.The Evidence-based Network for the Interpretation of Germline Mutant Alleles (ENIGMA) has recently developed specific criteria for interpreting the clinical significance, in relation to cancer risk, of constitutional variants of BRCA genes. These are available on the consortium website [27]. However, it must be considered that studies in mouse models have highlighted that not all BRCA gene variants associated with high risk of developing cancer are predictors of response to anti-PARP therapy [8].It is therefore necessary that laboratory reporting protocols, including the interpretation process, be made publicly available. In addition, the clinical significance of the BRCA gene variants identified should be clearly stated, along with a list of the essential information used for the classification [26,28]. In this regard, laboratories, in addition to taking part in external quality control programs, should contribute to a systematic and centralized collection of all BRCA variants observed with the aim to improve their classification [25].BRCA testing in the care & treatment pathwayThe use of BRCA testing as a treatment decision tool implies that it should be readily accessible to all those patients who may benefit from it and that the test results be made available within a time compatible with the clinical needs.The models of cancer genetic counseling developed in the preventive setting are currently insufficient to meet the increasing number of BRCA test requests, particularly when the test for genetic predisposition also has a predictive value for treatment which needs to be determined in a short time. The optimal model of genetic counseling within the prevention pathway involves detailed information and discussion of the genetic aspects right from the pretest stage. On the other hand, the need to obtain test results in a timely manner in order to implement treatment planning implies that also oncologists and gynecologists experienced in oncology can directly request the BRCA test to the laboratory. Even when the test is performed in the cancer treatment setting, the arrangement of full care pathways is mandatory, to ensure the correct interpretation of the results for clinical purposes, the correct way to manage family members at risk if a hereditary pathogenic variant is identified and the correct genetic assessment of cases with a noninformative BRCA test result [25–26,28].Physicians who request the BRCA test must receive appropriate updated training in genetic oncology. Since prescribers bear final responsibility for the use of the test result, they must verify that the laboratory uses appropriate protocols for correct clinical interpretation. Furthermore, protocols must be defined for the referral of patients with inherited pathogenic variant to a clinical cancer genetics team so that family members can receive appropriate care, as well as for particular cases warranting further genetic investigation [28].Each center must provide clear indications of the management pathways to the patients and their relatives, outlining the duties and responsibilities of the oncology team, of the laboratory and of the clinical cancer genetics team across the different phases of the defined care pathway. Should recognized standards not be available, one should consider submitting these pathways to verification via planned audits, with the aim to improve service quality.Essential items of the informed consentThe BRCA test for prediction of response to PARP inhibitors may be prescribed by clinical geneticists, oncologists and gynecologists with oncologic expertise. The prescriber has the responsibility to provide appropriate information to the patients on the genetic aspects associated with the results in order to enable them to make informed choices. The information provided to the patient should cover the potential therapeutic benefits of treatment with a PARP inhibitor, together with the possibility of detecting a high cancer risk condition for the patient and her relatives. The timing at which informed consent to genetic testing is obtained, as well as the modalities, must respect the will of the patient, who should be given the possibility to discuss all the different implications of genetic testing, such as whether or not to tell other family members about the test results, before taking the decision.Physicians who prescribe a BRCA test should abide to an appropriate communication and written informed consent collection protocol, using specific information material and consent forms. Oncologists and gynecologists with oncologic expertise who do not have experience in cancer genetics must follow a training program which includes ethical aspects of BRCA testing. Finally, the care pathway must clearly identify the cancer genetics team to be contacted, should the patient require a closer examination of the genetic aspects before deciding whether or not to undergo the test, as well as for those cases which present particular issues.ConclusionIn conclusion, BRCA mutations represent a biomarker predictive of sensitivity to treatment with PARP inhibitors, in addition to cancer risk assessment. As highlighted in this recommendation, several issues are associated with diagnostic BRCA testing: the screening methods vary across laboratories, and yet no standardized tests exist; BRCA analyses can reveal changes of unclear clinical significance, in addition to variants with a pathogenic or neutral role; finally, there is a need to devise the most appropriate report models, to avoid misinterpretations among clinicians and confusion among the patients. In this respect, the combined goal of this recommendation by the Italian scientific societies is to improve the outcome of therapy, to promote prevention and finally to reduce mortality of ovarian cancer.Financial & competing interests disclosureThe authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.No writing assistance was utilized in the production of this manuscript.Open accessThis work is licensed under the Creative Commons Attribution-NonCommercial 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/References1 Soegaard M, Kjaer SK, Cox M et al. BRCA1 and BRCA2 mutation prevalence and clinical characteristics of a population-based series of ovarian cancer cases from Denmark. Clin. Cancer Res. 14(12), 3761–3767 (2008).Crossref, Medline, CAS, Google Scholar2 Song H, Cicek MS, Dicks E et al. The contribution of deleterious germline mutations in BRCA1, BRCA2 and the mismatch repair genes to ovarian cancer in the population. Hum. Mol. Genet. 23(17), 4703–4709 (2014).Crossref, Medline, CAS, Google Scholar3 Alsop K, Fereday S, Meldrum C et al. BRCA mutation frequency and patterns of treatment response in BRCA mutation-positive women with ovarian cancer: a report from the Australian Ovarian Cancer Study Group. J. Clin. Oncol. 30(21), 2654–2663 (2012).Crossref, Medline, CAS, Google Scholar4 George J, Alsop K, Etemadmoghadam D et al. Nonequivalent gene expression and copy number alterations in high-grade serous ovarian cancers with BRCA1 and BRCA2 mutations. Clin. Cancer Res. 19(13), 3474–3484 (2013).Crossref, Medline, CAS, Google Scholar5 Ledermann J, Harter P, Gourley C et al. Olaparib maintenance therapy in patients with platinum-sensitive relapsed serous ovarian cancer: a preplanned retrospective analysis of outcomes by BRCA status in a randomised Phase 2 trial. Lancet Oncol. 15(8), 852–861 (2014).Crossref, Medline, CAS, Google Scholar6 Tcga: Integrated genomic analyses of ovarian carcinoma. Nature 474(7353), 609–615 (2011).Crossref, Medline, Google Scholar7 Bolton KL, Chenevix-Trench G, Goh C et al. Association between BRCA1 and BRCA2 mutations and survival in women with invasive epithelial ovarian cancer. JAMA 307(4), 382–390 (2012).Crossref, Medline, CAS, Google Scholar8 Drost R, Jonkers J. Opportunities and hurdles in the treatment of BRCA1-related breast cancer. Oncogene 33(29), 3753–3763 (2014).Crossref, Medline, CAS, Google Scholar9 Wiggans AJ, Cass GK, Bryant A, Lawrie TA, Morrison J. Poly(ADP-ribose) polymerase (PARP) inhibitors for the treatment of ovarian cancer. Cochrane Database Syst. Rev. (5), CD007929 (2015).Medline, Google Scholar10 Bouwman P, Jonkers J. Molecular pathways: how can BRCA-mutated tumors become resistant to PARP inhibitors? Clin. Cancer Res. 20(3), 540–547 (2014).Crossref, Medline, CAS, Google Scholar11 Kaelin WG Jr. The concept of synthetic lethality in the context of anticancer therapy. Nat. Rev. Cancer 5(9), 689–698 (2005).Crossref, Medline, CAS, Google Scholar12 Lord CJ, Tutt AN, Ashworth A. Synthetic lethality and cancer therapy: lessons learned from the development of PARP inhibitors. Annu. Rev. Med. 66, 455–470 (2015).Crossref, Medline, CAS, Google Scholar13 Michels J, Vitale I, Saparbaev M, Castedo M, Kroemer G. Predictive biomarkers for cancer therapy with PARP inhibitors. Oncogene 33(30), 3894–3907 (2014).Crossref, Medline, CAS, Google Scholar14 Curtin NJ. DNA repair dysregulation from cancer driver to therapeutic target. Nat. Rev. Cancer 12(12), 801–817 (2012).Crossref, Medline, CAS, Google Scholar15 Hilton JL, Geisler JP, Rathe JA, Hattermann-Zogg MA, Deyoung B, Buller RE. Inactivation of BRCA1 and BRCA2 in ovarian cancer. J. Natl Cancer Inst. 94(18), 1396–1406 (2002).Crossref, Medline, CAS, Google Scholar16 EMA: CHMP assessment report. www.ema.europa.eu/docs/en_GB/document_library/EPAR_-_Public_assessment_report/human/003726/WC500180154.pdf.Google Scholar17 Hogervorst FB, Nederlof PM, Gille JJ et al. Large genomic deletions and duplications in the BRCA1 gene identified by a novel quantitative method. Cancer Res. 63(7), 1449–1453 (2003).Medline, CAS, Google Scholar18 Kumps C, Van Roy N, Heyrman L et al. Multiplex amplicon quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma. BMC Genomics 11, 298 (2010).Crossref, Medline, Google Scholar19 Ellard S, Charlton R, Yau M et al. Clinical Molecular Genetics Society. Practice guidelines for Sanger sequencing analysis and interpretation. Birmingham, UK. www.acgs.uk.com/media/774807/bpg_for_targeted_next_generation_sequencing_may_2014_final.pdf.Google Scholar20 Grada A, Weinbrecht K. Next-generation sequencing: methodology and application. J. Invest. Dermatol. 133(8), e11 (2013).Crossref, Medline, Google Scholar21 Mardis ER. Next-generation DNA sequencing methods. Annu. Rev. Genomics Hum. Genet. 9, 387–402 (2008).Crossref, Medline, CAS, Google Scholar22 Meldrum C, Doyle MA, Tothill RW. Next-generation sequencing for cancer diagnostics: a practical perspective. Clin. Biochem. Rev. 32(4), 177–195 (2011).Medline, Google Scholar23 Rizzo JM, Buck MJ. Key principles and clinical applications of "next-generation" DNA sequencing. Cancer Prev. Res. (Phila.) 5(7), 887–900 (2012).Crossref, Medline, CAS, Google Scholar24 Walsh T, Lee MK, Casadei S et al. Detection of inherited mutations for breast and ovarian cancer using genomic capture and massively parallel sequencing. Proc. Natl Acad. Sci. USA 107(28), 12629–12633 (2010).Crossref, Medline, CAS, Google Scholar25 Wallis Y, Payne S, McAnulty C et al. Practice guidelines for the evaluation of pathogenicity and the reporting of sequence variants in clinical molecular genetics ACGS/VGKL. www.acgs.uk.com/media/774853/evaluation_and_reporting_of_sequence_variants_bpgs_june_2013_-_finalpdf.pdf.Google Scholar26 Plon SE, Eccles DM, Easton D et al. Sequence variant classification and reporting: recommendations for improving the interpretation of cancer susceptibility genetic test results. Hum. Mutat. 29(11), 1282–1291 (2008).Crossref, Medline, CAS, Google Scholar27 ENIGMA. https://enigmaconsortium.org/.Google Scholar28 Claustres M, Kozich V, Dequeker E et al. Recommendations for reporting results of diagnostic genetic testing (biochemical, cytogenetic and molecular genetic). Eur. J. Hum. Genet. 22(2), 160–170 (2014).Crossref, Medline, Google ScholarFiguresReferencesRelatedDetailsCited ByTumor BRCA Testing in Epithelial Ovarian Cancers: Past and Future—Five-Years' Single-Institution Experience of 762 Consecutive Patients23 March 2022 | Cancers, Vol. 14, No. 7Longitudinal and multi-tissue molecular diagnostics track somatic BRCA2 reversion mutations that correct the open reading frame of germline alteration upon clinical relapse22 February 2021 | npj Genomic Medicine, Vol. 6, No. 1Biologic behavior of resected BRCA-mutated pancreatic cancer: Comparison with sporadic pancreatic cancer and other BRCA-related cancersPancreatology, Vol. 21, No. 3Prevalence and Spectrum of BRCA Germline Variants in Central Italian High Risk or Familial Breast/Ovarian Cancer Patients: A Monocentric Study12 August 2020 | Genes, Vol. 11, No. 8Genetic testing for epithelial ovarian cancerBest Practice & Research Clinical Obstetrics & Gynaecology, Vol. 65An optimized BRCA1/2 next-generation sequencing for different clinical sample types1 January 2020 | Journal of Gynecologic Oncology, Vol. 31, No. 1BRCA germline mutation test for all woman with ovarian cancer?28 June 2019 | BMC Cancer, Vol. 19, No. 1BRCA1/2 Molecular Assay for Ovarian Cancer Patients: A Survey through Italian Departments of Oncology and Molecular and Genomic Diagnostic Laboratories9 October 2019 | Diagnostics, Vol. 9, No. 4Recommendations for the implementation of BRCA testing in ovarian cancer patients and their relativesCritical Reviews in Oncology/Hematology, Vol. 140Usefulness and Limitations of Comprehensive Characterization of mRNA Splicing Profiles in the Definition of the Clinical Relevance of BRCA1/2 Variants of Uncertain Significance1 March 2019 | Cancers, Vol. 11, No. 3The Era of PARP inhibitors in ovarian cancer: "Class Action" or not? A systematic review and meta-analysisCritical Reviews in Oncology/Hematology, Vol. 131Nipple-sparing bilateral prophylactic mastectomy and immediate reconstruction with TiLoop® Bra mesh in BRCA1/2 mutation carriers: A prospective study of long-term and patient reported outcomes using the BREAST-QThe Breast, Vol. 39Evaluation of a Streamlined Oncologist-Led BRCA Mutation Testing and Counseling Model for Patients With Ovarian CancerJournal of Clinical Oncology, Vol. 36, No. 13Main implications related to the switch to BRCA 1/2 tumor testing in ovarian cancer patients: a proposal of a consensus13 April 2018 | Oncotarget, Vol. 9, No. 28A Whole Germline BRCA2 Gene Deletion: How to Learn from CNV In Silico Analysis23 March 2018 | International Journal of Molecular Sciences, Vol. 19, No. 4Hereditary Breast and Ovarian Cancer Syndrome: Moving Beyond BRCA1 and BRCA2Advances in Anatomic Pathology, Vol. 25, No. 2Guidance Statement On BRCA1/2 Tumor Testing in Ovarian Cancer PatientsSeminars in Oncology, Vol. 44, No. 3Current perspectives on recommendations for BRCA genetic testing in ovarian cancer patientsEuropean Journal of Cancer, Vol. 69 Vol. 12, No. 18 eToC Sign up Follow us on social media for the latest updates Metrics History Published online 31 May 2016 Published in print September 2016 Information© Carmine PintoKeywords BRCA1 BRCA2 genetic testinggermline mutationsovarian cancerPARP inhibitorssomatic mutationsFinancial & competing interests disclosureThe authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.No writing assistance was utilized in the production of this manuscript.Open accessThis work is licensed under the Creative Commons Attribution-NonCommercial 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/PDF download
Referência(s)