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Characterization of a Renal Cell Carcinoma Cell Line Suitable as a Target for Immunological Studies in Vitro and in the NU/NU Mouse

1985; Lippincott Williams & Wilkins; Volume: 134; Issue: 6 Linguagem: Inglês

10.1016/s0022-5347(17)47710-x

1527-3792

Martin H. Goldrosen, Robert P. Huben, Glenn A. Miller, David A. Lewis, Hisako Ochi, Avery A. Sandberg, J. Edson Pontes,

Epigenetics and DNA Methylation

No AccessJournal of Urology1 Dec 1985Characterization of a Renal Cell Carcinoma Cell Line Suitable as a Target for Immunological Studies in Vitro and in the NU/NU Mouse Martin H. Goldrosen, Robert Huben, Glenn A. Miller, David A. Lewis, Hisako Ochi, Avery A. Sandberg, and J. Edson Pontes Martin H. GoldrosenMartin H. Goldrosen More articles by this author , Robert HubenRobert Huben More articles by this author , Glenn A. MillerGlenn A. Miller More articles by this author , David A. LewisDavid A. Lewis More articles by this author , Hisako OchiHisako Ochi More articles by this author , Avery A. SandbergAvery A. Sandberg More articles by this author , and J. Edson PontesJ. Edson Pontes More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)47710-XAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail A continuous human renal carcinoma cell line designated RPMI-SE was established from a patient with a poorly to moderately differentiated renal cell carcinoma. The cells are anchorage dependent, have a well defined globular shape with pseudopod-like structures, a doubling time in vitro of 24 hr. and are able to grow subcutaneously in female ICR Swiss nu/nu mice. RPMI-SE cells obtained from tissue culture and the nude mouse had the chromosome number of neartetraploidy. Common morphologic rearrangements were present in both the cell line and nu/nu mouse tumor. RPMI-SE was evaluated as a target cell line in an in vitro Indium-111 release assay. Three patterns of cytotoxicity were observed at an effector to target cell ratio of 100:L The highest degree of cytotoxicity (75 per cent) was obtained with autologous lymphocytes. An intermediate level of cytotoxicity (50 per cent) was obtained with allogeneic lymphocytes. The lowest level of cytotoxicity (27 per cent) was obtained with normal lymphocytes and was comparable to the level of cytotoxicity observed with the NK sensitive K562 target cell line. Morphologic data and chromosomal analysis indicate that the RPMI-SE cell line has maintained characteristics of the original tumor. This cell line will be useful for immunological studies as a target cell line in vitro as well as in vivo in the nude mouse. © 1985 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byKinoshita H, Ogawa O, Mitsumori K, Kakehi Y, Terachi T and Yoshida O (2018) LOW FREQUENCY OF POSITIVE TELOMERASE ACTIVITY IN A CHROMOPHOBE SUBTYPE OF RENAL CELL CARCINOMAJournal of Urology, VOL. 159, NO. 1, (245-251), Online publication date: 1-Jan-1998.Perrapato S, Slocum H, Huben R, Ghosh R and Rustum Y (2018) Assessment of Human Genitourinary Tumors and Chemosensitivity Testing In 3-Dimensional Collagen Gel CultureJournal of Urology, VOL. 143, NO. 5, (1041-1045), Online publication date: 1-May-1990. Volume 134Issue 6December 1985Page: 1271-1275 Advertisement Copyright & Permissions© 1985 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Martin H. Goldrosen More articles by this author Robert Huben More articles by this author Glenn A. Miller More articles by this author David A. Lewis More articles by this author Hisako Ochi More articles by this author Avery A. Sandberg More articles by this author J. Edson Pontes More articles by this author Expand All Advertisement PDF downloadLoading ...