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The Mechanism of Human Bladder Tumor Implantation in an in Vitro Model

1986; Lippincott Williams & Wilkins; Volume: 136; Issue: 2 Linguagem: Inglês

10.1016/s0022-5347(17)44926-3

1527-3792

Dov Pode, Y. Alon, Aviva T. Horowitz, Israël Vlodavsky, S Biran,

Urinary and Genital Oncology Studies

No AccessJournal of Urology1 Aug 1986The Mechanism of Human Bladder Tumor Implantation in an in Vitro Model Dov Pode, Yaakov Alon, Aviva T. Horowitz, Israel Vlodavsky, and Shoshana Biran Dov PodeDov Pode , Yaakov AlonYaakov Alon , Aviva T. HorowitzAviva T. Horowitz , Israel VlodavskyIsrael Vlodavsky , and Shoshana BiranShoshana Biran View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)44926-3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Implantation of tumor cells in the bladder following transurethral resection of superficial bladder tumors is believed to be one factor in the etiology of bladder tumor recurrences. Using an in vitro model system we have studied the initial interaction between bladder carcinoma cells and a naturally produced basement membrane-like substrate. Minced explants of superficial low grade human bladder tumors from 10 patients were plated into culture dishes coated with a naturally produced extracellular matrix (ECM). This ECM has been shown to resemble the human urothelial basement membrane and submucosa in its macromolecular composition and ultrastructural appearance. It was found that a firm attachment of the human bladder tumor cells occurred within one hour, reached a maximal value within 24 hours and was followed by flattening and proliferation of the plated cells. These results indicate that prevention of tumor implantation should be initiated in the first hour after transurethral resection of the bladder tumors. This assay can be used for the investigation of various treatments to prevent tumor implantation. © 1986 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byCusano J, Haddock P, Jackson M, Luk M, Wiener S, Cox A and Meraney A (2015) Process Improvements Positively Impact the Use of Intravesical Mitomycin C after Transurethral Resection of Nonmuscle Invasive Bladder Cancer in a Large, Urban Urology PracticeUrology Practice, VOL. 2, NO. 6, (291-297), Online publication date: 1-Nov-2015.Ham W, Kim W, Jeon H, Lee D and Choi Y (2009) Long-Term Outcome of Simultaneous Transurethral Resection of Bladder Tumor and Prostate in Patients With Nonmuscle Invasive Bladder Tumor and Bladder Outlet ObstructionJournal of Urology, VOL. 181, NO. 4, (1594-1599), Online publication date: 1-Apr-2009.BROCKS C, BÜTTNER H and BöHLE A (2018) INHIBITION OF TUMOR IMPLANTATION BY INTRAVESICAL GEMCITABINE IN A MURINE MODEL OF SUPERFICIAL BLADDER CANCERJournal of Urology, VOL. 174, NO. 3, (1115-1118), Online publication date: 1-Sep-2005.TSIVIAN A, SHTRICKER A and SIDI A (2018) Simultaneous Transurethral Resection of Bladder Tumor and Benign Prostatic Hyperplasia: Hazardous or A Safe Timesaver?Journal of Urology, VOL. 170, NO. 6, (2241-2243), Online publication date: 1-Dec-2003.BÖHLE A, JURCZOK A, ARDELT P, WULF T, ULMER A, JOCHAM D and BRANDAU S (2018) INHIBITION OF BLADDER CARCINOMA CELL ADHESION BY OLIGOPEPTIDE COMBINATIONS IN VITRO AND IN VIVOJournal of Urology, VOL. 167, NO. 1, (357-363), Online publication date: 1-Jan-2002.Fleischmann J, Park M and Hassan M (2018) Fibronectin Expression on Surgical Specimens Correlated with the Response to Intravesical Bacillus Calmette-Guerin TherapyJournal of Urology, VOL. 149, NO. 2, (268-271), Online publication date: 1-Feb-1993.See W, Rohlf D and Crist S (2018) In Vitro Particulate Adherence to Fibronectin: Correlation with in Vivo Particulate Adherence to Sites of Bladder InjuryJournal of Urology, VOL. 147, NO. 5, (1416-1423), Online publication date: 1-May-1992.See W and Williams R (2018) Urothelial Injury and Clotting Cascade Activation: Common Denominators in Particulate Adherence to Urothelial SurfacesJournal of Urology, VOL. 147, NO. 3 Part 1, (541-548), Online publication date: 1-Mar-1992.Grossman H, Liebert M, Wedemeyer G, Wilson G and Flint A (2018) Fibronectin Distribution in Normal and Malignant UrotheliumJournal of Urology, VOL. 143, NO. 2, (418-420), Online publication date: 1-Feb-1990.Pan J, Slocum H, Rustum Y, Greco W, Gaeta J and Huben R (2018) Inhibition of Implantation of Murine Bladder Tumor by Thiotepa in Cauterized BladderJournal of Urology, VOL. 142, NO. 6, (1589-1593), Online publication date: 1-Dec-1989.Ratliff T, Kavoussi L and Catalona W (2018) Role of Fibronectin in Intravesical BCG Therapy for Superficial Bladder CancerJournal of Urology, VOL. 139, NO. 2, (410-414), Online publication date: 1-Feb-1988.Pobe D, Horowitz A, Vlodavsky I, Shapiro A and Biran S (2018) Prevention of Human Bladder Tumor Cell Implantation in an in Vitro AssayJournal of Urology, VOL. 137, NO. 4, (777-781), Online publication date: 1-Apr-1987. Volume 136 Issue 2 August 1986 Page: 482-486 Advertisement Copyright & Permissions© 1986 by The American Urological Association Education and Research, Inc.Metrics Author Information Dov Pode More articles by this author Yaakov Alon More articles by this author Aviva T. Horowitz More articles by this author Israel Vlodavsky More articles by this author Shoshana Biran More articles by this author Expand All Advertisement PDF downloadLoading ...