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First Report of Little cherry virus 1 (LChV-1) in Sweet Cherry in Spain

2016; American Phytopathological Society; Volume: 100; Issue: 11 Linguagem: Inglês

10.1094/pdis-05-16-0620-pdn

1943-7692

Ana Belén Ruiz-García, Cristina Martinez, Rogelio Santiago, Michelle Garcia, N. de Prado, Antonio Olmos,

Plant Pathogenic Bacteria Studies

HomePlant DiseaseVol. 100, No. 11First Report of Little cherry virus 1 (LChV-1) in Sweet Cherry in Spain PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Little cherry virus 1 (LChV-1) in Sweet Cherry in SpainA. B. Ruiz-García, C. Martínez, R. Santiago, M. T. García, N. de Prado, and A. OlmosA. B. Ruiz-García, C. Martínez, R. Santiago, M. T. García, N. de Prado, and A. OlmosAffiliationsAuthors and Affiliations A. B. Ruiz-García C. Martínez , Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain R. Santiago , Servicio de Sanidad Vegetal de Badajoz, 06071 Badajoz, Spain M. T. García , Servicio de Sanidad Vegetal de Plasencia, 10600 Plasencia, Cáceres, Spain N. de Prado , Estación de Avisos Agrícolas del Bierzo, 24549 Carracedelo, León, Spain A. Olmos , Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain. Published Online:12 Aug 2016https://doi.org/10.1094/PDIS-05-16-0620-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Little cherry virus 1 (LChV-1) is a member of the genus Velarivirus of the family Closteroviridae. LChV-1 was first identified by Keim-Konrad and Jelkmann (1996). LChV-1, along with Little cherry virus 2 (LChV-2), has been associated to little cherry disease, whose symptoms include dramatic reduction of fruit size, color, and taste. Although LChV-1 is associated to this symptomatology in susceptible cultivars, in other varieties the infection produces moderate symptoms or is symptomless. LChV-2 seems to be responsible for little cherry symptoms in most cases (Jelkmann and Eastwell 2011). In spring 2012, 21 samples of sweet cherry shoots from D.O. Valle del Jerte (Cáceres) and five similar samples from Ponferrada (León) showing different symptoms, such as fruit deformation, reduced fruit size, and red coloration of leaves, were analyzed by RT-PCR using specific primers designed on LChV-1 sequences found on the databases (5′-CCAATGCACAAAGCACATATGA-3′ and 5′-ACCGCGACGTGGTCCTAATA-3′). In 17 samples from Jerte and five samples from Ponferrada, the specific 140-bp RT-PCR product was obtained. Two of these positive samples, one from Jerte and one from Ponferrada, were selected for further analysis by next generation sequencing of siRNAs using TrueSeq Illumina technology on a HiSeq 1000 system. De novo assembly was performed using Geneious software. BlastN and TblastX analyses of contigs confirmed the presence of LChV-1 in both samples and revealed the possible presence of other viruses and a viroid (Apple mosaic virus, Prunus necrotic ringspot virus, Prune dwarf virus, Cherry virus A, and Hop stunt viroid in the Jerte sample, and Prune dwarf virus and Cherry virus A in the Ponferrada sample). A near full-length LChV-1 genome of 16,933 nt was recovered for both isolates (GenBank accession nos. KX192366 and KX192367) by the extension of the contigs and by mapping the Illumina reads on reference genomes deposited on GenBank. The full-length genome sequences of the two spanish LChV-1 isolates showed a high similarity with the Taian isolate, recently described in China (Wang et al. 2016), with total percentage identities of 97.9 and 98%, respectively. A total identity of 98.1% was found between Jerte and Ponferrada isolates. This is the first report of LChV-1 in sweet cherry in Spain. The prevalence of the virus in the cherry growing areas in the country remains to be determined.References:Jelkmann, W., and Eastwell, K. C. 2011. Page 153 in: Virus and Virus-Like Diseases of Pome and Stone Fruits. A. Hadidi et al., eds. APS Press, St. Paul, USA. Link, Google ScholarKeim-Konrad, R., and Jelkmann, W. 1996. Arch. Virol. 141:1437. https://doi.org/10.1007/BF01718246 Crossref, ISI, Google ScholarWang, J., et al. 2016. Arch. Virol. 161:749. https://doi.org/10.1007/s00705-015-2737-z Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 11 November 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 7 Oct 2016Published: 12 Aug 2016First Look: 6 Jun 2016Accepted: 2 Jun 2016 Pages: 2340-2340 Information© 2016 The American Phytopathological SocietyCited byMolecular characterisation of little cherry virus 1 infecting apricots in the Czech Republic15 July 2020 | European Journal of Plant Pathology, Vol. 158, No. 1High-Throughput Sequencing Assists Studies in Genomic Variability and Epidemiology of Little Cherry Virus 1 and 2 infecting Prunus spp. in Belgium29 June 2019 | Viruses, Vol. 11, No. 7Prunus avium (Sweet cherry)6 June 2020Recent Advances on Detection and Characterization of Fruit Tree Viruses Using High-Throughput Sequencing Technologies17 August 2018 | Viruses, Vol. 10, No. 8High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics21 July 2018 | Viruses, Vol. 10, No. 7Development of a Real-Time RT-PCR for the Universal Detection of LChV1 and Study of the Seasonal Fluctuation of the Viral Titer in Sweet Cherry CultivarsAsimina T. 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