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[HLA-DRB1 alleles genotyping in patients with rheumatoid arthritis in Chinese].

1997; National Institutes of Health; Volume: 36; Issue: 2 Linguagem: Inglês

Yongqiang Zhao, Youhai Dong, Fengying Tang,

Monoclonal and Polyclonal Antibodies Research

To explore the role of HLA-DRB1 genes in the development of rheumatoid arthritis (RA) and the correlations between HLA-DR alleles and clinical manifestations of patients with RA we studied 86 patients and 106 race matched controls in whom HLA-DR typing was performed by the method of DNA amplification with sequence-specific primers (PCR-SSP). The subtypes of HLA-DR4 were determined by the method of hybridization of PCR products with sequence-specific oligonucletides (PCR-SSO). The absence or presence of HLA-DR4 and its subtypes was evaluated with the clinical and serological characteristics of the patients. Compared with controls, an increased gene frequency of HLA-DR4 (48.8% vs 17.9%, P < 0.001) and a decreased frequency of HLA-DR5 (16.3% vs 27.4%, P = 0.06) were found. The DRB1 * 0405 accounted for 61.9% of DR4+ RA patients and 21.1% of DR+4 controls (P < 0.01). There was no difference between the DR4+ and DR4- patients with respect to age, sex, duration of disease, extra-articular manifestations including secondary Sjogren's syndrome. But rheumatoid factor (RF) was associated with HLA-DR4 (P < 0.05). According to the X-ray stage, the patients of DR4+ were more severe than those of DR4- (P < 0.05). HLA-DR4 and DR4 subtype of DRB1 * 0405 were related to the development of RA in Chinese. HLA-DR4 can be a useful prognostic marker in the patients with RA.