Secretion of interleukin-1 by bovine milk macrophages
1991; American Veterinary Medical Association; Volume: 52; Issue: 6 Linguagem: Inglês
10.2460/ajvr.1991.52.06.858
ISSN1943-5681
AutoresI. Politis, B.W. McBride, J.H. Burton, Xin Zhao, Joseph D. Turner,
Tópico(s)T-cell and Retrovirus Studies
ResumoSUMMARY The relative sensitivity of bovine blood monocytes and macrophages isolated from milk to lipopolysaccharide, with respect to interleukin 1 ( il -1) production, was evaluated. Addition of lipopolysaccharide (0 to 30 μg/ml) to the culture medium resulted in increases in secreted and intracellular il -1 activity for monocytes and milk macrophages, with maximal stimulation achieved at 30 μg of lipopolysaccharide/ ml of medium. At this concentration of lipopolysaccharide, monocytes released 76% of the total il -1, whereas milk macrophages released only 26% of the total il -1 produced within the cell. Secretion of a small quantity of il -1 was a common property of macrophages isolated from healthy and mastitic quarters. We concluded that limited secretion of il -1 may render the milk macrophages less efficient in promoting lymphocyte activation.
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