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Reduced brain edema after traumatic brain injury in mice deficient in P-selectin and intercellular adhesion molecule-1

2000; Oxford University Press; Volume: 67; Issue: 2 Linguagem: Inglês

10.1002/jlb.67.2.160

1938-3673

Michael J. Whalen, Timothy M. Carlos, C. Edward Dixon, Paul Robichaud, Robert S. B. Clark, Donald W. Marion, Patrick M. Kochanek,

Traumatic Brain Injury and Neurovascular Disturbances

Abstract Platelet (P-) selectin and intercellular adhesion molecule-1 (ICAM-1) mediate accumulation of neutrophils in brain. However, the mechanisms regulating neutrophil accumulation and damage after traumatic brain injury (TBI) are poorly defined. We hypothesized that mice deficient in both P-selectin and ICAM-1 (-/–) would have decreased brain neutrophil accumulation and edema, and improved functional and histopathological outcome after TBI compared with wild-type (+/+). In Protocol I, neutrophils and brain water content were quantified at 24 h after TBI. No difference in brain neutrophil accumulation was observed between groups; however, brain edema was decreased in dual P-selectin and ICAM-1 –/– (P < 0.05 vs. +/+ mice). In Protocol II, after TBI, tests of motor and memory function and histopathology were assessed over 21 days. No difference in motor or memory function or histopathological damage was observed between +/+ and –/– mice. A role for adhesion molecules in the pathogenesis of brain edema independent of leukocyte accumulation in brain is suggested. J. Leukoc. Biol. 67: 160–168; 2000.