Portal de Periódicos da CAPES

Elektronenoptische Charakterisierung menschlicher Plasmalipoproteine nach Isolierung mittels zonaler Ultrazentrifugation

1976; Thieme Medical Publishers (Germany); Volume: 101; Issue: 44 Linguagem: Inglês

10.1055/s-0028-1104311

1439-4413

Josef R. Patsch, S. Sailer, H Braunsteiner, Trudy Forte,

Blood disorders and treatments

Human plasma proteins were isolated by zonal ultracentrifugation and the fractions obtained examined under the electron microscope. HDL2 had a high degree of uniformity in normal persons, characterised by numerous two-dimensional, hexagonal arrangements. Optical diffraction analysis of HDL2 gave a centre distance of 11.3 nm, individual HDL2 particles being spherical with a diameter of 9.8 nm. HDL3 represented a somewhat less uniform lipoprotein population with a particle diameter between 6.5 and 9.5 nm. LDL had a diameter of 20-25 nm. In patients with hyperlipoproteinaemia type III a third peak was isolated, in addition to the two normal lipoprotein peaks VLDL and LDL. It lies between them and has been called Lp III. VLDL from type III plasma consisted of spherical particles, diameter 31-81 nm, morphologically indistinguishable from normal VLDL. Lp III consisted of a relatively homogeneous spherical lipoprotein population with a diameter of 25-30 nm. LDL consisted of spherical particles whose size was highly homogeneous (18-23 nm diameter), indistinguishable from normal LDL.