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Electrophoretic study of the genome of human rotavirus from Maceió, Brazil.

1986; Associação Brasileira de Divulgação Científica; Volume: 19; Issue: 1 Linguagem: Inglês

1414-431X

Houly Ca, Uchoa Mm, Zaidan Am, A Gomes-Neto, de-Oliveira Fm, Athayde Ma, Almeida Mf, Pereira Hg,

Animal Virus Infections Studies

Rotaviruses were detected by enzyme immunoassay (EIA) in 53 (13.3%) of 397 fecal samples from children with acute gastroenteritis in the city of Maceio, Alagoas, Brazil. Polyacrylamide gel electrophoretic (PAGE) patterns characteristic of rotavirus double-stranded RNA were detected in 51 (96.2%) of the 53 EIA-positive samples. Of the RNA-positive samples, 1 (2%) was classified as subgroup 1 (short profile), 49 (96%) as subgroup 2 (long profile) and 1 (2%) could not be classified because of the absence of bands 10 and 11. The strains of subgroup 2 showed a great degree of electrophoretic heterogeneity and could be divided into several subcategories. Two samples showed splitting of one of the genome segments. PAGE, a very sensitive method capable of identifying rotavirus RNA genomes, has demonstrated that human rotaviruses detected in Maceio present many differences in RNA electrophoretic patterns.