TT-seq maps the human transient transcriptome
2016; American Association for the Advancement of Science; Volume: 352; Issue: 6290 Linguagem: Inglês
10.1126/science.aad9841
ISSN1095-9203
AutoresBjörn Schwalb, Margaux Michel, Benedikt Zacher, Katja Frühauf, Carina Demel, Achim Tresch, Julien Gagneur, Patrick Cramer,
Tópico(s)RNA modifications and cancer
ResumoTT-Seq maps a transient transcriptome RNA expression is related to protein abundance and cellular function. However, the amounts of RNA generated at any one time-point have been difficult to determine. Schwalb et al. developed a method, transient transcriptome sequencing (TT-Seq), to collect and sequence all RNA segments synthesized over 5 minutes. Because 5 minutes is not long enough to fully degrade even the most transient RNA, this method can detect the synthesis of most RNA without bias. Applying this method to human K562 cells, TT-Seq detected thousands of noncoding transcripts, providing a snapshot of RNA synthesis rates and RNA half-lives, and full-length maps of short-lived RNAs such as enhancers and short intergenic noncoding RNAs. Science , this issue p. 1225
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