Variability of 11q23 rearrangements in hematopoietic cell lines identified with fluorescence in situ hybridization
1993; Elsevier BV; Volume: 81; Issue: 11 Linguagem: Inglês
10.1182/blood.v81.11.3027.3027
ISSN1528-0020
AutoresHirofumi Kobayashi, Rafael Espinosa, MJ Thirman, Davis Em, MO Diaz, MM Le Beau, JD Rowley,
Tópico(s)Genomic variations and chromosomal abnormalities
ResumoWe mapped and ordered 17 cosmid, phage, and plasmid clones to chromosome 11, bands q22-q24, using fluorescence in situ hybridization (FISH). We then analyzed four hematopoietic cell lines with 11q23 rearrangements, Karpas 45, SUP-T13, RC-K8, and Karpas 422, using these probes. The studies showed that the translocation breakpoints of the Karpas 45 and SUP-T13 cell lines, which were derived from T-cell malignancies, were located in the same breakpoint cluster region of the MLL gene as the RS4; 11 cell line and patients with the t(9;11), t(11;19), and t(6;11) described previously. We confirmed that the translocation breakpoint of the RC-K8 cell line was located telomeric to the MLL gene, and found that the derivative 11 chromosome of the Karpas 422 cell line, which had been thought to contain a t(4;11) (q21;q23), was in fact formed through a deletion and an inverted tandem repeat of part of 11q.
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