Portal de Periódicos da CAPES

Estrogen regulation of JE/MCP-1 mRNA expression in fibroblasts

1996; Oxford University Press; Volume: 59; Issue: 4 Linguagem: Inglês

10.1002/jlb.59.4.562

1938-3673

Elizabeth J. Kovacs, Douglas E. Faunce, D S Ramer-Quinn, Francis J. Mott, Peter Dy, Michelle R. Frazier-Jessen,

Endometriosis Research and Treatment

Abstract We have recently demonstrated that 17β-estradiol (E2) inhibits peritoneal adhesion formation. Because macrophages play a central role in inflammation and wound healing, we chose to investigate whether the E2 could inhibit the expression of JE, the murine monocyte chemoattractant protein-1 (MCP-1). To accomplish this, murine fibroblasts were cultured with physiological concentrations of E2 (3–300 pg/ml) with or without inducers of JE/MCP-1 mRNA expression. Untreated cells failed to express the message, but, following stimulation, a marked increase in JE/MCP-1 mRNA expression was observed. At 10–30 pg/ml, E2 had no effect on JE/MCP-1 mRNA expression in stimulated fibroblasts. In contrast, lower and higher doses of E2 inhibited the expression of JE/MCP-1 mRNA in stimulated fibroblasts. Treatment with tamoxifen reversed the E2-inhibition of expression of the message. These data demonstrate that JE/MCP-1 mRNA expression is controlled, in part, by estrogen and suggest that macrophage recruitment may be affected by circulating levels of E2.