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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Analysis of polymorphisms in codons 11, 72 and 248 of TP53 in Brazilian women with breast cancer

2016; Research Foundation of Ribeirão Preto; Volume: 15; Issue: 1 Linguagem: Inglês

10.4238/gmr.15017055

ISSN

1676-5680

Autores

Bruna Cristine de Almeida, J.P.F.O. Kleine, C.M. Camargo-Kosugi, Mário Roberto Pontes Lisboa, Carolina Nunes França, Jerônimo Pereira de França, I. D. C. G. Silva,

Tópico(s)

Molecular Biology Techniques and Applications

Resumo

The association between TP53 gene polymorphisms and breast cancer (BC) in Brazilian women is a controversial topic.In this cross-sectional study, we evaluated the association between clinical pathological variables and three polymorphisms (TP53*11, TP53*72, and TP53*248) in BC patients and controls.Genomic DNA was extracted from the blood cells of 393 participants; the cancer-free control subjects were 26-72 years old (41 ± 11.03) and the BC patients were 28-80 years old (51 ± 10.70).We used standard polymerase chain reaction-restriction fragment length polymorphism and confirmed the results by genetic sequencing.In TP53*11, there was 100% homozygous Glu distribution in both groups.

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