
Analysis of polymorphisms in codons 11, 72 and 248 of TP53 in Brazilian women with breast cancer
2016; Research Foundation of Ribeirão Preto; Volume: 15; Issue: 1 Linguagem: Inglês
10.4238/gmr.15017055
ISSN1676-5680
AutoresBruna Cristine de Almeida, J.P.F.O. Kleine, C.M. Camargo-Kosugi, Mário Roberto Pontes Lisboa, Carolina Nunes França, Jerônimo Pereira de França, I. D. C. G. Silva,
Tópico(s)Molecular Biology Techniques and Applications
ResumoThe association between TP53 gene polymorphisms and breast cancer (BC) in Brazilian women is a controversial topic.In this cross-sectional study, we evaluated the association between clinical pathological variables and three polymorphisms (TP53*11, TP53*72, and TP53*248) in BC patients and controls.Genomic DNA was extracted from the blood cells of 393 participants; the cancer-free control subjects were 26-72 years old (41 ± 11.03) and the BC patients were 28-80 years old (51 ± 10.70).We used standard polymerase chain reaction-restriction fragment length polymorphism and confirmed the results by genetic sequencing.In TP53*11, there was 100% homozygous Glu distribution in both groups.
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