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Decreased expression of tumor suppressive miR-874 and its clinical significance in human osteosarcoma

2015; Research Foundation of Ribeirão Preto; Volume: 14; Issue: 4 Linguagem: Inglês

10.4238/2015.december.23.19

1676-5680

L.Q. Zhang, Shengjie Sun, W.Y. Li, Zitong Feng, Xiaochao Xu, Q. Zhuang, Jun Fang,

Cancer-related molecular mechanisms research

Dysregulation of microRNAs (miRs) is associated with cancer development and progression and aberrant expression of miR-874 have been found in some types of cancer.However, the expression and function of miR-874 in osteosarcoma remain unclear.The aim of this study was to explore the effects of miR-874 in osteosarcoma tumorigenesis and development.The expression level of miR-874 was quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR) in human osteosarcoma cell lines and tissues.Using a miR-874 mimic, cell proliferation and migration assays were performed in an osteosarcoma cell line and tumorigenicity was observed in vivo in order to determine the effects of miR-874 in osteosarcoma cell lines and tissues.MiR-874 was significantly downregulated in osteosarcoma cell lines and clinical L.Q.Zhang et al. ©FUNPEC-RP www.funpecrp.com.brGenetics and Molecular Research 14 (4): 18315-18324 (2015) specimens.Decreased miR-874 expression was significantly associated with large tumor size, distant metastasis, and advanced clinical stage, and was an independent predictor of poor survival.Overexpression of miR-874 inhibited cell proliferation, invasion and migration in vitro, promoted cell apoptosis in vitro, and suppressed tumorigenicity in vivo.These findings indicate that miR-874 may act as a tumor suppressor in osteosarcoma and could serve as a novel therapeutic agent for miRbased therapy.