Portal de Periódicos da CAPES

Transferability of Microsatellite Markers in the Betulaceae

2010; American Society for Horticultural Science; Volume: 135; Issue: 2 Linguagem: Inglês

10.21273/jashs.135.2.159

2327-9788

Kahraman Gürcan, Shawn A. Mehlenbacher,

Plant and Fungal Interactions Research

Microsatellite-containing sequences for the Betulaceae ( Betula , Corylus , and Alnus ) were retrieved from GenBank and used to develop twelve new microsatellite marker primer pairs that amplified and were polymorphic in european hazelnut ( Corylus avellana ). The primer pairs were characterized using 50 european hazelnut accessions. Nine of these microsatellites that segregated in a mapping population were assigned to linkage groups. The 12 new primer pairs will be useful in genetic studies in Corylus and Betula . To investigate transferability of microsatellite primer pairs in the family Betulaceae, we assessed the ability of 129 simple sequence repeat (SSR) primer pairs (75 from Corylus , 52 from Betula , and two from Alnus ) to amplify DNA of 69 accessions representing diverse taxa. Microsatellite primer pairs from Betula amplified 92% of Betula , 51% of Alnus , 41% of Corylus , 37% of Carpinus , 35% of Ostryopsis , and 34% of Ostrya acce ssions. In the 69 accessions, microsatellite primer pairs from Corylus amplified 81% of Corylus , 55% of Carpinus , 53% of Ostrya , 51% of Ostryopsis , 41% of Alnus , and 39% of Betula accessions. An additional 147 SSR primer pairs developed from Corylus , used to amplify a subset of 32 accessions, gave similar values: 92% in Corylus , 33% in Carpinus , 33% in Ostrya , 44% in Ostryopsis , 35% in Alnus , and 54% in Betula . The high transferability (>39%) of microsatellite primer pairs between Betula and Corylus will allow comparative studies of the two genera with the greatest economic importance.