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Molecular Characterization of Parapoxviruses from Infected Wild Japanese Serows (<i>Capricornis crispus</i>) in Ishikawa Prefecture(Virology)

2009; Volume: 14; Issue: 2 Linguagem: Inglês

10.5686/jjzwm.14.103

2185-744X

Mika Ito, Yûji Hayakawa, Eiichi Shintani, Yasuo Inoshima,

Virus-based gene therapy research

Cases of suspected parapoxvirus infection were observed in wild Japanese serows (Capricornis crispus) in different areas of Hakusan in Ishikawa Prefecture in 2008. Polymerase chain reaction (PCR) and sequence analyses were conducted, and a specific 594-bp fragment was amplified by parapoxvirus-specific PCR from the lesions of two serows from distinct areas within the Prefecture. Parapoxviruses were isolated from the lesions of two serows and were identified as orf virus, designated IJS081 and IJS087. Partial sequences of the viral envelope gene were determined from the isolated IJSO81 and IJS087. The sequences were identical to each other and to the orf virus S-1 strain that was isolated from a Japanese serow in the neighboring Gifu Prefecture in 1985. These results suggest that the infections in Japanese serows in different areas of Hakusan were caused by the same or genetically similar orf virus. Moreover, the sequence of the viral envelope gene in orf viruses in Japanese serows in this area seems to have been stable for more than 20 years.