Contribution of "in vitro" assays to preclinical and premarketing testing in immunotoxicology.
1993; National Institute of Public Health; Volume: 1; Issue: 2 Linguagem: Inglês
ISSN
1803-1048
Autores Tópico(s)Animal testing and alternatives
ResumoAn attempt to replace the vivo testing by vitro exposure of cells to drugs is discussed from the point of view of the limitations of these artificial test conditions. The critical reminders are evaluated, the most important ones being the pharmacokinetics, pharmacodynamics and metabolism of drugs in the body what may be a cause of the discrepancy between vivo and vitro exposure assays. Various sensitivity of different cell types and of different phases of the generation cycle represent the crucial importance in the immune response and in the drug-cell interactions. The vitro assays are technically sophisticated and mostly based on the tissue-culture techniques. However, both in immunology and immunotoxicology standardization, validation and usage of rules of Good Laboratory Practice are required. Finally, possibilities and contribution of vitro assays to immunotoxicology are listed. It is apparent that for establishment of common rules these studies require intensive international and interlaboratory cooperation and coordination.
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