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Characterization of soluble dietary fiber from Moringa oleifera seeds and its immunomodulatory effects

2016; Elsevier BV; Volume: 91; Linguagem: Inglês

10.1016/j.ijbiomac.2016.06.013

1879-0003

Sandanamudi Anudeep, Vaddi K. Prasanna, S Adya, Cheruppanpullil Radha,

Protein Hydrolysis and Bioactive Peptides

Moringa oleifera (moringa or drumstick) seeds are a potential source of dietary fiber with 6.5% w/w soluble dietary fiber. Biochemical characterization of moringa seed soluble fiber revealed that it is a glycoprotein with 5% neutral sugars. Arabinose and xylose are the major neutral sugars identified by gas liquid chromatography (GLC). Moringa seed soluble fiber was identified as protease resistant⿿glycoprotein and termed as moringa seed resistant protein (MSRP). MSRP was found to be a homodimer (18 kDa) containing two 9 kDa monomeric units as revealed by SDS-PAGE analysis with pI 10.8. Immunostimulating activity of MSRP was assessed by murine splenocyte proliferation and production of NO from macrophages. MSRP at low concentration (0.01 μg/well) strongly increased proliferation of splenocytes, while MSRP at high concentration weakly responded. MSRP induced 6-fold increase in NO production when compared to the control which indicates the activation of macrophages. MSRP isolated from defatted moringa seed flour is a potent mitogen, enhancing the proliferation of lymphocytes and inducing NO from macrophages. This study concludes that moringa seed is a potential nutritional source to promote the immune system of the host.