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Development of a Radioimmunoassay for Human Group-II Phospholipase A2 and Demonstration of Postoperative Elevation

1991; Karger Publishers; Volume: 45; Issue: 4 Linguagem: Inglês

10.1159/000468890

2504-2564

Yasuki Matsuda, Michio Ogawa, Kiyoshi Sakamoto, Shin-ichi Yamashita, A. Kanda, Masaki KOHNO, Nobuo Yoshida, Junichi Nishijima, Asako Murata, Takahiro Mori,

Peptidase Inhibition and Analysis

A radioimmunoassay (RIA) for the determination of human group-II phospholipase A(2) (M-PLA(2)) has been developed. M-PLA(2) was purified from human spleen. Monoclonal antibody (IgG) was prepared by fusion of splenic cells from immunized mice with M-PLA(2) and the mouse myeloma cell line NS-1. The RIA was carried out by a single antibody method. The assay is sensitive (0.78 μg/1), reproducible and specific. In healthy individuals, the serum M-PLA2 concentration ranges from 1.4 to 4.2 μg/l, the average being 2.2 ± 0.1 μg/1 (mean ± SE). Using the RIA, we found increased serum M-PLA(2) in patients with various infections and malignant tumors. We also showed the postoperative transient elevation of serum M-PLA(2) in cases without any infectious complications. The elevation was independent of the surgical procedure or site. The maximum serum M-PLA(2) level was seen on the 2nd to 4th postoperative day. In these patients, the serum M-PLA(2) and C-reactive protein levels were significantly correlated. The present study indicated that serum M-PLA(2) is an acute phase reactant.