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Antimicrobial Activities of Indole Alkaloids from Tabernaemontana catharinensis

2011; SAGE Publishing; Volume: 6; Issue: 2 Linguagem: Inglês

10.1177/1934578x1100600209

1934-578X

Maria Rita Furquini Medeiros, Luiz Afonso de Melo Prado, Vanessa C. Fernandes, Sérgio Souza Figueiredo, Juliana da Silva Coppede, Juliana Simões Martins, Giovana Maria Lanchoti Fiori, Nilce Maria Martinez-Rossi, Renê Oliveira Beleboni, Sílvia Helena Taleb Contini, Paulo Sérgio Pereira, Ana Lúcia Fachin,

Pharmacological Effects of Natural Compounds

Tabernaemontana catharinensis root bark ethanol extract, EB2 fraction and the MMV alkaloid (12-methoxy-4-methylvoachalotine) were evaluated for their antimicrobial activities. T. catharinensis ethanol extract was effective against both strains of the dermatophyte Trichophyton rubrum at concentrations of 2.5 mg/mL (wild strain) and 1.25 mg/mL (mutant strain), while the EB2 fraction and MMV alkaloid showed a strong antifungal activity against wild and mutant strains with MIC values of <0.02 and 0.16 mg/mL, respectively. The EB2 fraction showed a strong antibacterial activity against ATCC strains of S. aureus, S. epidermidis, E. coli and P. aeruginosa with MICs from <0.02 to 0.04 mg/mL, as well as against resistant clinical isolates species of Enterococcus sp, Klebsiella oxytoca, Citrobacter, K. pneumoniae, P. mirabilis, S. aureus, S. epidermidis, E. coli and P. aeruginosa with MIC values ranging from 0.04 to 0.08 mg/mL. The MMV alkaloid presented a MIC of 0.16 mg/mL against the strains of S. aureus and E. coli ATCC. For the resistant clinical isolates Enterococcus sp, Citrobacter, S. aureus, S. epidermidis, E. coli and P. aeruginosa the MIC of MMV ranged from 0.08 to 0.31 mg/mL. The chromatography analysis of the EB2 fraction revealed the presence of indole alkaloids, including MMV, possibly responsible for the observed antimicrobial activity.