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Monoclonal Antibodies to Human Urothelial Cell Lines and Hybrids: Production and Characterization

1985; Lippincott Williams & Wilkins; Volume: 133; Issue: 3 Linguagem: Inglês

10.1016/s0022-5347(17)49048-3

1527-3792

L K Trejdosiewicz, Jennifer Southgate, Jennifer A. Donald, John R. Masters, Peter J. Hepburn, G. M. Hodges,

Celiac Disease Research and Management

No AccessJournal of Urology1 Mar 1985Monoclonal Antibodies to Human Urothelial Cell Lines and Hybrids: Production and Characterization L.K. Trejdosiewicz, J. Southgate, J.A. Donald, J.R.W. Masters, P.J. Hepburn, and G.M. Hodges L.K. TrejdosiewiczL.K. Trejdosiewicz More articles by this author , J. SouthgateJ. Southgate More articles by this author , J.A. DonaldJ.A. Donald More articles by this author , J.R.W. MastersJ.R.W. Masters More articles by this author , P.J. HepburnP.J. Hepburn More articles by this author , and G.M. HodgesG.M. Hodges More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)49048-3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Eleven independent monoclonal antibodies, the LBS series, were isolated after immunization of mice with RT112 cells, a continuous cell line derived from a transitional cell carcinoma of the human bladder. These antibodies were tested by indirect immunofluorescence on a panel of 28 human cell lines, of which 17 were urothelial carcinoma-derived, 4 of non-urothelial carcinoma origin, 3 fibroblast cell lines, 4 lymphoblastoid lines and 7 murine cell lines. Also tested were 7 somatic cell hybrid clones derived by fusion of human RT112 cells with murine bladder carcinoma MB63T/H cells. None of the LBS antibodies reacted with mesenchyme-derived cells, although all reacted with RT112 cells. On the basis of reactivity with the cell line panel, the antibodies were divided into 3 groups. Group I (LBS-1 and 19) reacted with all human epithelium-derived cell lines. Group II (LBS-2, 8, 15 and 17) reacted only with human urothelium-derived cells, tending to recognise the least anaplastic types. Group III antibodies (LBS-10, 20A, 20B, 21 and 34) were urothelium-specific on the human continuous cell line panel, but additionally reacted with murine urothelial and epithelial cell lines. The 6 human-specific antibodies (Group I and II) were used for preliminary analysis of human gene expression in a series of 7 mouse × human urothelial somatic cell hybrids. Each hybrid reacted with at least 1 LBS antibody, although there were changes in gene expression with time in culture, indicating both loss and unmasking of human genes. These data suggest the LBS-series antibodies recognise different determinants associated with epithelial and urothelial cell differentiation, and thus may be valuable probes in the study of normal differentiation and malignant transformation in human urothelial cells. © 1985 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByHuland E, Huland H, Meier T, Baricordi O, Fradet Y, Grossman H, Hodges G, Messing E and Schmitz-Draeger B (2018) Comparison of 15 Monoclonal Antibodies against Tumor-Associated Antigens of Transitional Cell Carcinoma of the Human BladderJournal of Urology, VOL. 146, NO. 6, (1631-1636), Online publication date: 1-Dec-1991.Takahashi N, Takahashi S, Takahashi K, Suzuki T and Funyu T (2018) A Monoclonal Antibody to Human Transitional Cell Carcinoma of the Bladder: Production and CharacterizationJournal of Urology, VOL. 138, NO. 1, (207-211), Online publication date: 1-Jul-1987.Bander N (2018) Monoclonal Antibodies: State of the ArtJournal of Urology, VOL. 137, NO. 4, (603-612), Online publication date: 1-Apr-1987.Arndt R, Dürkopf H, Huland H, Donn F, Loening T and Kalthoff H (2018) Monoclonal Antibodies for Characterization of the Heterogeneity of Normal and Malignant Transitional CellsJournal of Urology, VOL. 137, NO. 4, (758-763), Online publication date: 1-Apr-1987. Volume 133Issue 3March 1985Page: 533-538 Advertisement Copyright & Permissions© 1985 by The American Urological Association Education and Research, Inc.MetricsAuthor Information L.K. Trejdosiewicz More articles by this author J. Southgate More articles by this author J.A. Donald More articles by this author J.R.W. Masters More articles by this author P.J. Hepburn More articles by this author G.M. Hodges More articles by this author Expand All Advertisement Loading ...