Portal de Periódicos da CAPES

Applications of Yeast Surface Display for Protein Engineering

2015; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-4939-2748-7_8

1940-6029

Gerald Maxwell Cherf, Jennifer R. Cochran,

Fungal and yeast genetics research

The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. Additional applications of yeast display are also briefly presented, including protein epitope mapping, identification of protein-protein interactions, and uses of displayed proteins in industry and medicine.