
A MLST Clade 2 Clostridium difficile strain with a variant TcdB induces severe inflammatory and oxidative response associated with mucosal disruption
2016; Elsevier BV; Volume: 40; Linguagem: Inglês
10.1016/j.anaerobe.2016.06.005
ISSN1095-8274
AutoresCecília Leite Costa, Diana López‐Ureña, Thiago de Oliveira Assis, Ronaldo A. Ribeiro, Rodrigo Otávio Silveira Silva, Maja Rupnik, Mark H. Wilcox, Alex F. Carvalho, Anderson Oliveira do Carmo, Adriana Abalen Martins Dias, Cibele Barreto Mano de Carvalho, Esteban Chaves-Olarte, César Rodríguez, Carlos Quesada-Gómez, Gerly Anne de Castro Brito,
Tópico(s)Helicobacter pylori-related gastroenterology studies
ResumoThe epidemiology of Clostridium difficile infections is highly dynamic as new strains continue to emerge worldwide. Here we present a detailed analysis of a new C. difficile strain (ICC-45) recovered from a cancer patient in Brazil that died from severe diarrhea. A polyphasic approach assigned a new PCR-ribotype and PFGE macrorestriction pattern to strain ICC-45, which is toxigenic (tcdA(+), tcdB(+) and ctdB(+)) and classified as ST41 from MLST Clade 2 and toxinotype IXb. Strain ICC-45 encodes for a variant TcdB that induces a distinct CPE in agreement with its toxinotype. Unlike epidemic NAP1/027 strains, which are also classified to MLST Clade 2, strain ICC-45 is susceptible to fluoroquinolones and does not overproduce toxins TcdA and TcdB. However, supernatants from strain ICC-45 and a NAP1/027 strain produced similar expression of pro-inflammatory cytokines, epithelial damage, and oxidative stress response in the mouse ileal loop model. These results highlight inflammation and oxidative stress as common features in the pathogenesis of C. difficile Clade 2 strains. Finally, this work contributes to the description of differences in virulence among various C. difficile strains.
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