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The Effects of Short-term In-vivo Ischemia on the Contractile Function of the Rabbit Urinary Bladder

1988; Lippincott Williams & Wilkins; Volume: 139; Issue: 6 Linguagem: Inglês

10.1016/s0022-5347(17)42917-x

1527-3792

Harcharan Gill, Frederick C. Monson, Alan J. Wein, Michael R. Ruggieri, Robert M. Levin,

Urological Disorders and Treatments

No AccessJournal of Urology1 Jun 1988The Effects of Short-term In-vivo Ischemia on the Contractile Function of the Rabbit Urinary Bladder Harcharan S. Gill, Frederick C. Monson, Alan J. Wein, Michael R. Ruggieri, and Robert M. Levin Harcharan S. GillHarcharan S. Gill , Frederick C. MonsonFrederick C. Monson , Alan J. WeinAlan J. Wein , Michael R. RuggieriMichael R. Ruggieri , and Robert M. LevinRobert M. Levin View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)42917-XAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail The proper functioning of any smooth muscle requires adequate perfusion with oxygen and nutrients. Ischemia compromises both these factors and results in dysfunction, the extent depending on the degree and duration of ischemia. This study determined the effects of one, two and four weeks in vivo ischemia on the capacity, compliance and contractile function of the rabbit urinary bladder. Morphological changes were also studied with light microscopy. Different degrees of ischemia were achieved as follows. In the unilateral group the vesical artery was tied on one side and the animals were sacrificed at one week or two weeks. In the bilateral group the vesical arteries on both sides were tied and the animals were sacrificed one week later. In the bilateral staged group the vesical artery was tied on one side and after one week the contralateral artery was ligated, and the animals sacrificed one week after the second procedure. Muscle strips were studied for contractile response, with a distinction being made between the ipsilateral and contralateral side of vessel ligation in the unilateral group. The results were as follows. 1) In the unilateral group there was a 72% reduction in the contractile response of the dome of the bladder to bethanecol on the side of vessel ligation and a 32% reduction on the contralateral side. The response to methoxamine on the base was reduced by 44%, with no difference between the ipsilateral and contralateral side. 2) Bilateral vessel ligation resulted in a 97% reduction in contractile response to bethanechol on the dome and a 75% in the response of the base to methoxamine. 3) Staged bilateral ligation resulted in a 69% reduction in the contractile response of the dome to bethanechol and a 18% reduction in the response of the base to methoxamine. Ischemia caused a marked reduction in the compliance and capacity of the bladder in all the three groups, with the most marked changes in the bilateral group. Multiple spontaneous contractions were noted in the three groups during the filling phase of the cystometrograms. Histological features correlated well with the functional changes in the different groups. © 1988 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByKim J, Jang H, Bae J and Lee J (2018) Effects of Coenzyme Q10 on Bladder Dysfunction Induced by Chronic Bladder Ischemia in a Rat ModelJournal of Urology, VOL. 189, NO. 6, (2371-2376), Online publication date: 1-Jun-2013.Hashitani H, Takano H, Fujita K, Mitsui R and Suzuki H (2018) Functional Properties of Suburothelial Microvessels in the Rat BladderJournal of Urology, VOL. 185, NO. 6, (2382-2391), Online publication date: 1-Jun-2011.Chang I, Oh S and Kim S (2018) A Possible Relationship Between Testosterone and Lower Urinary Tract Symptoms in MenJournal of Urology, VOL. 182, NO. 1, (215-220), Online publication date: 1-Jul-2009.LEVIN R and HUDSON A (2018) THE MOLECULAR GENETIC BASIS OF MITOCHONDRIAL MALFUNCTION IN BLADDER TISSUE FOLLOWING OUTLET OBSTRUCTIONJournal of Urology, VOL. 172, NO. 2, (438-447), Online publication date: 1-Aug-2004.Bajory Z, Hutter J, Krombach F and Messmer K (2018) The Role of Endothelin-1 in Ischemia-Reperfusion Induced Acute Inflammation of the Bladder in RatsJournal of Urology, VOL. 168, NO. 3, (1222-1225), Online publication date: 1-Sep-2002.KOZLOWSKI R, KERSHEN R, SIROKY M, KRANE R and AZADZOI K (2018) CHRONIC ISCHEMIA ALTERS PROSTATE STRUCTURE AND REACTIVITY IN RABBITSJournal of Urology, VOL. 165, NO. 3, (1019-1026), Online publication date: 1-Mar-2001.BRATSLAVSKY G, KOGAN B and LEVIN R (2018) URETHRA IS MORE SENSITIVE TO ISCHEMIA THAN BLADDER: EVIDENCE FROM AN IN VITRO RAT STUDYJournal of Urology, VOL. 165, NO. 6 Part 1, (2086-2090), Online publication date: 1-Jun-2001.Greenland J and Brading A (2018) THE EFFECT OF BLADDER OUTFLOW OBSTRUCTION ON DETRUSOR BLOOD FLOW CHANGES DURING THE VOIDING CYCLE IN CONSCIOUS PIGSJournal of Urology, VOL. 165, NO. 1, (245-248), Online publication date: 1-Jan-2001.AZADZOI K, TARCAN T, KOZLOWSKI R, KRANE R and SIROKY M (2018) OVERACTIVITY AND STRUCTURAL CHANGES IN THE CHRONICALLY ISCHEMIC BLADDERJournal of Urology, VOL. 162, NO. 5, (1768-1778), Online publication date: 1-Nov-1999.SAITO M and MIYAGAWA I (2018) DIRECT DETECTION OF NITRIC OXIDE IN RAT URINARY BLADDER DURING ISCHEMIA-REPERFUSIONJournal of Urology, VOL. 162, NO. 4, (1490-1495), Online publication date: 1-Oct-1999.AZADZOI K, TARCAN T, SIROKY M and KRANE R (2018) ATHEROSCLEROSIS-INDUCED CHRONIC ISCHEMIA CAUSES BLADDER FIBROSIS AND NON-COMPLIANCE IN THE RABBITJournal of Urology, VOL. 161, NO. 5, (1626-1635), Online publication date: 1-May-1999.Chen M, Buttyan R and Levin R (2018) Genetic and Cellular Response to Unilateral Ischemia of the Rabbit Urinary BladderJournal of Urology, VOL. 155, NO. 2, (732-737), Online publication date: 1-Feb-1996.Batista J, Wagner J, Azadzoi K, Krane R and Siroky M (2018) Direct Measurement of Blood Flow in the Human BladderJournal of Urology, VOL. 155, NO. 2, (630-633), Online publication date: 1-Feb-1996.Tammela T, Longhurst P, Wein A and Levin R (2018) The Effect of Furosemide-Induced Diuresis on Rabbit Micturition and Bladder Contractile FunctionJournal of Urology, VOL. 150, NO. 1, (204-208), Online publication date: 1-Jul-1993.Eika B, Levin R, Monson F, Murphy M and Longhurst P (2018) 3H-Thymidine Uptake by the Rat Urinary Bladder After Induction Of Diabetes MellitusJournal of Urology, VOL. 150, NO. 4, (1316-1320), Online publication date: 1-Oct-1993.Tong-Long Lin A, Monson F, Kato K, Haugaard N, Wein A and Levin R (2018) Effect of Chronic Ischemia on Glucose Metabolism of Rabbit Urinary BladderJournal of Urology, VOL. 142, NO. 4, (1127-1133), Online publication date: 1-Oct-1989. Volume 139Issue 6June 1988Page: 1350-1354 Advertisement Copyright & Permissions© 1988 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Harcharan S. Gill More articles by this author Frederick C. Monson More articles by this author Alan J. Wein More articles by this author Michael R. Ruggieri More articles by this author Robert M. Levin More articles by this author Expand All Advertisement PDF DownloadLoading ...