Performance Characteristics of a New Hybrid Quadrupole Time-of-Flight Tandem Mass Spectrometer (TripleTOF 5600)
2011; American Chemical Society; Volume: 83; Issue: 13 Linguagem: Inglês
10.1021/ac200812d
ISSN1520-6882
AutoresGenna Andrews, Brigitte Simons, J. Bryce Young, Adam M. Hawkridge, David C. Muddiman,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoThe TripleTOF 5600 System, a hybrid quadrupole time-of-flight mass spectrometer, was evaluated to explore the key figures of merit in generating peptide and protein identifications that included spectral acquisition rates, data quality, proteome coverage, and biological depth. Employing a Saccharomyces cerevisiae tryptic digest, careful consideration of several performance features demonstrated that the speed of the TripleTOF contributed most to the resultant data. The TripleTOF system was operated with 8, 20, and 50 MS/MS events in an effort to compare with other MS technologies and to demonstrate the abilities of the instrument platform.
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