Artigo Revisado por pares

Proliferation and Na+/H+ Antiport Activity in Human Fibroblasts From Type I Diabetic Patients With Nephropathy

1993; Oxford University Press; Volume: 6; Issue: 2 Linguagem: Inglês

10.1093/ajh/6.2.170

ISSN

1941-7225

Autores

Karen Soussan, Eric Dausse, Thierry Hannedouche, José Timsit, Florence Lemoult, Christian Boîtard, Jean‐Pierre Grünfeld, Pascale Guicheney,

Tópico(s)

Birth, Development, and Health

Resumo

To investigate whether constitutive alterations of the Na+ / H+ antiport or of cell proliferation control mechanisms are implicated in development of nephropathy in insulin-dependent diabetics (IDD), skin fibroblasts from controls, recent-onset IDD with normal or high glomerular filtration rates, and IDD with proteinuria were cultured by explant technique. The Na+ / H+ antiport activity was studied using the pH sensitive fluorescent dye BCECF. The cell growth capacity was investigated by determination of cell DNA doubling time and by nuclear [3H]thymidine incorporation in response to serum. The Na+ / H+ antiport activity and fibroblast growth capacity did not differ between fibroblasts from controls and IDD patients, suggesting the absence of a genetic alteration of the Na+ / H+ antiport in the development of diabetic nephropathy. Am J Hypertens 1993;6:170-173

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