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Proliferation and Na+/H+ Antiport Activity in Human Fibroblasts From Type I Diabetic Patients With Nephropathy

1993; Oxford University Press; Volume: 6; Issue: 2 Linguagem: Inglês

10.1093/ajh/6.2.170

1941-7225

Karen Soussan, Eric Dausse, Thierry Hannedouche, José Timsit, Florence Lemoult, Christian Boîtard, Jean‐Pierre Grünfeld, Pascale Guicheney,

Birth, Development, and Health

To investigate whether constitutive alterations of the Na+ / H+ antiport or of cell proliferation control mechanisms are implicated in development of nephropathy in insulin-dependent diabetics (IDD), skin fibroblasts from controls, recent-onset IDD with normal or high glomerular filtration rates, and IDD with proteinuria were cultured by explant technique. The Na+ / H+ antiport activity was studied using the pH sensitive fluorescent dye BCECF. The cell growth capacity was investigated by determination of cell DNA doubling time and by nuclear [3H]thymidine incorporation in response to serum. The Na+ / H+ antiport activity and fibroblast growth capacity did not differ between fibroblasts from controls and IDD patients, suggesting the absence of a genetic alteration of the Na+ / H+ antiport in the development of diabetic nephropathy. Am J Hypertens 1993;6:170-173