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In vitro expression of the alpha and beta subunits of the Na,K-ATPase.

1988; National Institutes of Health; Volume: 268B; Linguagem: Inglês

Maureen Gilmore-Hebert, R W Mercer, Jay W. Schneider, E J Benz,

Ion Transport and Channel Regulation

Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.