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Characterization of heavy metals resistant yeast isolated from activated sludge in Rungkut, Surabaya, Indonesia as biosorbent of mercury, copper, and lead

2016; American Institute of Physics; Linguagem: Inglês

10.1063/1.4953535

1935-0465

Wahyu Irawati, Yantra Wijaya, Salomo Christian, Eric Santoso Djojo,

Chromium effects and bioremediation

One of the common problems that occur in industrial sites in Indonesia is heavy metal pollution. It is important to study the indigenous microorganisms in heavy metal polluted sites because it may provide microorganisms under unfavorable conditions which could be exploited in bioremediation processing. Isolate ES10.4 was a yeast resistant heavy metals isolated from the sewage of industrial Rungkut in Surabaya. The purpose of this research was to study the potency of isolate ES10.4 as biosorbent of copper, mercury, cadmium, and lead. The growth was observed by measuring the optical density of cells using a spectrophotometer. The resistance of isolate ES10.4 to the heavy metals was determined by measuring the MIC (Minimum Inhibitory Concentration). Biosorption of copper and lead were determined by atomic absorption spectrophotometer. Meanwhile, the biosorbtion of mercury was measured by mercury evaporation unit. The results showed that isolate ES10.4 has multiple resistances to heavy metals. The MICs of this isolate to copper, mercury, cadmium, and lead were 13 mM, 1.5 mM, 0.3 mM, and 17 mM, respectively. Isolate ES10.4 was able to remove lead up to 25.36 % and 29.55 % in medium containing 12 mM and 14 mM of lead, respectively. The isolate was also able to remove copper and mercury up to 30.61% and 35.20% from medium supplemented with 13 mM CuSO4.5H2O and 1.5 mM HgCl2 after 24 h, respectively. Yeast isolate ES10.4 had high heavy metal resistance and the ability to reduce of copper, mercury, and lead, therefore, may be applicable for the treatment of heavy metals containing waste water.