Artigo Revisado por pares

Impaired Akt phosphorylation in B-cells of patients with common variable immunodeficiency

2016; Elsevier BV; Volume: 175; Linguagem: Inglês

10.1016/j.clim.2016.09.009

ISSN

1521-7035

Autores

Reza Yazdani, Mazdak Ganjalikhani‐Hakemi, Mohammadhosein Esmaeili, Hassan Abolhassani, Shahram Vaeli, Abbas Rezaei, Zohre Sharifi, Gholamreza Azizi, Nima Rezaei, Asghar Aghamohammadi,

Tópico(s)

Immune Cell Function and Interaction

Resumo

Common variable immunodeficiency (CVID) is a heterogeneous group of primary immunodeficiency characterized by recurrent infections. We evaluated whether defective PI3K/Akt/FoxO pathway could influence B-cell fate. Determination of B-cell subsets in CVD patients and healthy donors (HDs) were performed using flow cytometry. We evaluated mRNA and protein expression of PI3K, Akt and FoxO using real-time PCR and flow cytometry, respectively. Moreover, phosphorylated Akt (pAkt) expression in B-cells has been measured by flowcytometry. We identified a significant reduction in the percentage of marginal zone like B-cells, memory B-cells (total, switched and unswitched) and plasmablasts in patients, as these decreased B-cell subsets had a significant negative correlation with increased apoptosis in patients. Surprisingly, we identified decreased pAkt expression in B-cells of patients than HDs. We described for the first time impaired pAkt expression in B-cells of CVID patients that had a significant correlation with antibody response to the vaccine and worse clinical complications.

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