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BIBTEX RIS

Nanostructured SBA-15 silica: An effective protective vehicle to oral hepatitis B vaccine immunization

2016; Elsevier BV; Volume: 12; Issue: 8 Linguagem: Inglês

10.1016/j.nano.2016.06.003

1549-9642

Karina Scaramuzzi, Gabriela D. Tanaka, Francisco Mariano Neto, Paulo R. A. F. Garcia, Joel José Megale Gabrili, Denise Cristina André Oliveira, Denise V. Tambourgi, Juliana Sekeres Mussalem, Danielle Paixão-Cavalcante, M. T. D. Orlando, Viviane Fongaro Botosso, Cristiano L. P. Oliveira, Márcia Carvalho de Abreu Fantini, Osvaldo A. Sant’Anna,

Glycosylation and Glycoproteins Research

Due to its physicochemical properties, nanostructured mesoporous SBA-15 silica shows great potential as a vaccine adjuvant. This study evaluated the capacity of SBA-15 to encapsulate/adsorb the recombinant purified HBsAg from the Hepatitis B virus and the immunoresponsiveness of mice orally immunized with HBsAg inside SBA-15. A simulation of small angle X-ray scattering experimental results, together with the nitrogen adsorption isotherms data, allowed to determine the appropriate mass ratio of HBsAg:SBA-15, indicating antigen encapsulation into SBA-15 macroporosity. This was also evaluated by bicinchoninic acid assay and gel electrophoresis. The recruitment of inflammatory cells, an increase in production of specific antibodies, and the non-influence of silica on TH1 or TH2 polarization were observed after oral immunization. Besides, SBA-15 enhanced the phagocytosis of ovalbumin by dendritic cells, an important key to prove how this adjuvant works. Thus, it seems clear that the nanostructured SBA-15 is an effective and safe adjuvant for oral immunizations.