Effective Antisense Gene Regulation via Noncationic, Polyethylene Glycol Brushes
2016; American Chemical Society; Volume: 138; Issue: 29 Linguagem: Inglês
10.1021/jacs.6b05787
ISSN1943-2984
AutoresXueguang Lu, Fei Jia, Xuyu Tan, Dali Wang, Xueyan Cao, Jiamin Zheng, Ke Zhang,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoNegatively charged nucleic acids are often complexed with polycationic transfection agents before delivery. Herein, we demonstrate that a noncationic, biocompatible polymer, polyethylene glycol, can be used as a transfection vector by forming a brush polymer-DNA conjugate. The brush architecture provides embedded DNA strands with enhanced nuclease stability and improved cell uptake. Because of the biologically benign nature of the polymer component, no cytotoxicity was observed. This approach has the potential to address several long-lasting challenges in oligonucleotide therapeutics.
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